MECHANISTIC STUDIES OF MOTONEURON DEATH

描述：本提案中概述的实验旨在检验 运动神经元死亡的生化和分子机制 发展。拟议的研究将集中于澄清一些 调节神经元死亡的细胞内事件以及死亡是如何 神经元可能会向非神经元细胞发出信号，让它们对其死亡做出反应。这项工作将 为主要调查者的研究重点提供基础 新实验室。三个具体目标是：(1)隔离和 鉴定新的神经元死亡基因；(2)分离和鉴定 介导运动神经元死亡的冰样基因和，(3)分离和 描述垂死运动神经元的生化和分子变化 这可能标志着吞噬细胞清除了它们的碎片。可以预期的是， 了解调节神经元的细胞内和细胞间过程 死亡将有助于我们对中枢神经系统发育的基本理解 过程以及对发生的细胞死亡的洞察 在受伤后或在神经退行性疾病中。

DESCRIPTION: Experiments outlined in this proposal are intended to examine the biochemical and molecular mechanisms of motor neuron death during development. The proposed research will focus on elucidating some of the intracellular events that mediate neuronal death as well as how the dying neuron may signal non-neuronal cells to respond to its death. The work will provide the foundation for the research focus of the principal investigators new laboratory. The three specific aims are to: (1) isolate and characterize novel neuronal death genes; (2) to isolate and characterize ICE-like genes that mediate motor neuron death and, (3) to isolate and characterize biochemical and molecular changes within dying motor neurons that may signal phagocytic removal of their debris. It can be expected that understanding the intra- and intercellular processes that regulate neuron death will contribute to our understanding of basic CNS developmental processes as well as provide insights into the cell death that occurs following injury or in neurodegenerative diseases.