MU OPIOID RECEPTOR/G PROTEIN RELATIONSHIP IN VIVO

MU阿片受体(MOR)是镇痛和神经递质的重要中介。 鸦片类药物的奖励/成瘾特性。所有阿片受体 是7个跨膜区G蛋白超家族的成员吗？ 感受器。阿片类配体一般对日晒有抑制作用 变速箱。然而，MOR被认为能够与 大量的G蛋白α亚基。这项提议的目的是为了 MOR与G-蛋白α在体内相互作用的研究 亚单位。由于BITH MOR和G-蛋白α亚基表现出 选择性的细胞和亚细胞定位，我提出了一个解剖学的 如果它们是区域性的、细胞性的和亚细胞性的，则接近于确定 NOR与G蛋白共定位的特异性。在……里面 此外，免费的生物化学培训将更直接地解决 天气共生代表了一种功能上的互动。 此外，由于阿片类信号通路是用 关系和依赖的发展，我建议tp决定是否 我观察到随着慢性疾病MOR和G-蛋白变化的关系 鸦片类药物治疗。

The mu opioid receptor (MOR) is akey mediator of the analgesic and rewarding/addictive properties of opiate drugs. All opioid receptors are members of surperfamily of 7 transmembrance-domain G-protein coucled receptors. Opioid ligands generally have inhibitory effects on sunaptic transmission. However, MOR is thought to be able to interact with numerous G-protein alpha subunits. The goal of this proposal is to gain insight into the vivo interactions between MOR and G-protein alpha subunits. Since bith MOR and G-protein alpha subunits demonstrate a selective cellular and subcellular localization, I propose an anatomical apprach to detemine if their is regional, cellular, and subcellular specilicity in the colocalization between NOR and G-protein. In addition a complimentary biochemical apprach will more directly address weather colocailization represents a functional interaction. Furthermore, since opiate signaling pathways are modified with the development of toloerance and dependence, I propose tp determine if the relationships I observe bewteen MOR and G-proteins change with chronic opiate drug treatment.