BONE REGENERATION ON SURFACE-MODIFIED POLYMERIC IMPLANTS

生物材料的两个主要领域代表了生物医学产业 和骨科社区面临着巨大的挑战， 用于关节置换和组织工程。 大多数人的无能 用于骨科植入物的材料，以与 骨通常与松动和失效有关。 这个，还有 对组织工程的兴趣日益浓厚， 技术适用于关键的生物医学应用， 注意探索使用专有的表面活化和 微孔泡沫技术，开发独特的基板，以实现 关键聚合物植入物的组织再生 因此，主要 该计划第一阶段的目标是确定 实现骨再生的表面磷酸化 聚丙烯（PP）、聚乙烯（PE）和聚丙交酯/乙交酯（PLG） 具有和不具有微孔表皮的基材，当放置在6至8 作为山羊股骨的经皮质植入物。 因此，第一阶段需要 (1)可吸收PLG的制备：（2）PLG、PE的转化 和PP到可植入棒;（3）在一个上形成微孔皮肤 （4）表面膦酰化和表征 具有和不具有微孔皮肤的棒;（5）植入表面改性的 如5中的PLG棒，其已经用细胞附着物处理 因子;和（7）进行组织学检查和推出试验 以确定骨再生和/或 向内生长和骨/植入物界面结合强度。 阶段结果 我的研究将用于第二阶段计划的设计，其中包括 进行（1）详细的动物研究， 高分子量聚乙烯（PE-PE）骨植入物;（2） 开发选定的骨科器械并完成安全性 种植体原型的研究;（3）体内外研究 独特的磷酸化微孔PLG的应用研究 共聚物作为骨再生的支架。 建议的商业应用：成功应用 表面膦酰化技术与一种简单的 预期形成的微孔和/或表面微纹理植入物 对无水泥的发展有直接的，积极的影响， 具有杂化骨/植入物界面的聚合物骨假体。这 预计将（1）立即应用于高负荷和低负荷- 人工关节和硬组织表面置换的轴承部件，以及 (2)提供了一个新的动力，追求发展纤维增强 聚合物复合材料作为金属替代品在几个骨科 植入物.

Two major areas of biomaterials which present the biomedical industry and orthopedic community with a formidable challenge pertain to their use in joint replacement and tissue engineering. Inability of most materials used in orthopedic implants to form an interfacial bond with bone is often associated with loosening and failure. This, and the growing interest in tissue engineering and the great promise this technology holds for use in critical biomedical applications directed our attention to explore the use of proprietary surface activation and microcellular foam technologies to develop unique substrates to achieve tissue regeneration about key polymeric implants. Thus, the primary objective of Phase I of this program is to determine the feasibility of achieving bone regeneration about surface-phosphonylated polypropylene (PP), polyethylene (PE), and polylactide/glycolide (PLG) substrates with and without microporous skin, when placed at 6 to 8 weeks as transcortical implants in goat femur. Thus, Phase I entails (1) the preparation of an absorbable PLG; (2) conversion of PLG, PE and PP to implantable rods; (3) formation of microporous skin on one set of rods; (4) surface phosphonylation and characterization of the rods with and without microporous skin; (5) implanting surface modified PLG rods as in 5, which have been treated with a cell attachment factor; and (7) conducting a histological examination and push-out tests of the retrieved femur segments to determine bone regeneration and/or ingrowth, and bone/implant interfacial bond strength. Results of Phase I study will be used in the design of Phase II plans which include conducting (1) detailed animal study on optimally surface-modified ultrahigh molecular weight polyethylene (UHMW-PE) bone implants; (2) development of a selected orthopedic device and completing safety study on an implant prototype; and (3) pursuing in vitro and in vivo studies on the sue of unique phosphonylated microporous PLG copolymers as scaffold for bone regeneration. PROPOSED COMMERCIAL APPLICATION: Successful application of the surface phosphonylation technology in conjunction with an easily formed microporous and/or surface-microtextured implant is expected to have an immediate, positive impact on the development of cementless, polymeric bone prostheses with hybridized bone/implant interface. This is expected to (1) find immediate application in high and low load- bearing components of artificial joints and hard tissue resurfacing, and (2) provide a new incentive to pursue development of fiber-reinforced polymeric composites as substitutes for metals in several orthopedic implants.