NEW METHOD FOR MEASUREMENT OF CDT LEVEL IN SERUM

描述：(改编自申请者的摘要)创新 旨在提供一种新的方法，用于 人血中缺糖转铁蛋白(CDT)的AS检测 酒精滥用的生化标志。CTD已经被广泛地证明是 是长期过量饮酒的敏感和特异标记物 消费。用于CTD测量的现有分析技术 在血液中或其对完整转铁蛋白的配给中涉及分离 从其他血液蛋白中提取CTD，然后进行浓度测量 通常使用免疫分析方法。这些技术一般都是 耗时、昂贵，而且必须由训练有素的专业人员执行。 Analiza正在提出一种新的技术来测量 CDT/转铁蛋白比值直接用血样检测而不用前处理 分离。这项技术是基于水的一种新应用 两阶段划分。这项技术是独一无二的 获得的信息是廉价的、省时省力的，以及 可能提供现有不可能提供的附加信息 技术。第一阶段的工作将包括设计和分析 试验及其初步可行性和验证研究。第二期及第二期 III的工作将集中在广泛的验证协议上，一起 随着优化研究和原型的自动构建 乐器。

DESCRIPTION: (Adapted from the applicant's abstract) The innovation described in this proposal is aimed at providing a new method for detection of carbohydrate-deficient transferrin (CDT) in human blood as a biochemical marker of alcohol abuse. CTD has been extensively shown to be a sensitive and specific marker related to long term excessive alcohol consumption. Existing analytical techniques for the measurements of CTD in the blood or its ration to intact transferrin involve separation of CTD from other blood proteins, followed by concentration measurement typically using immunoassay methods. These techniques are in general time consuming, expensive, and must be performed by trained professionals. ANALIZA is proposing a new technique for the measurement of CDT/transferrin ratio directly using blood samples without pre- separation. The technique is based upon a novel application of aqueous two-phase partitioning. The technique is unique in the type of information obtained, is inexpensive and time-and labor-efficient, and may provide additional information not possible with existing technologies. Phase I work will involve the design and analysis of the test and its preliminary feasibility and validation study. Phases II and III work will concentrate on a wide-range validation protocol, together with optimization studies and a construction of a prototype automated instrument.