SOMATIC STEM CELL REGULATION IN THE DROSOPHILA GERMARIUM

本研究的长期目标是了解 生长、分化和图案化过程在体内发生， 果蝇生殖器中的体干细胞增殖（位于 每个卵巢管的尖端）作为模型系统。以前的实验 提出了一个独特的作用，刺猬（hh）在过程中。因此 确定生殖室中体细胞增殖是否 绝对依赖于hh，并且在需要其表达的情况下，FLP- OUT技术将用于生成空HH克隆。来识别细胞 响应于hh，将在野生型中分析标记的细胞克隆。 对比hh--过表达的生殖细胞。此外，两个备选方案 方法（分子或遗传筛选）被提出来鉴定 参与控制体细胞增殖的其他基因 生殖器 进一步分析分离基因的潜在作用 在对照组中，体细胞增殖将在 这一研究金期限，并继续在稍后阶段。 使用这种 一个强大遗传系统来识别控制细胞的因子 在体内的增殖分化，并研究其作用机制 将是有价值的，以了解如何破坏这种监管 过程导致病理状况，例如癌症，并且可以 提供治疗策略的见解。

The long term objective of the research proposed here is to understand how growth, differentiation and patterning processes take place in vivo, using somatic stem cell proliferation in the Drosophila germarium (located at the tip of each ovariole) as a model system. Previous experiments have suggested a unique role for hedgehog (hh) in process. Therefore, to determine whether somatic cell proliferation in the germarium is absolutely dependent on hh and where its expression is necessary, the FLP- out technique will be used to generate null hh clones. To identify cells responsive to hh, marked clones of cells will be analyzed in wild-type versus hh--overexpressing germarium. In addition, two alternative approaches (a molecular or a genetic screen) are proposed to identify other genes involved in the control of somatic cell proliferation in the germarium. Further analysis of the potential role of the isolated genes in the control somatic cell proliferation will be initiated during the term of this fellowship and continued at a later stage. The use of this powerful genetic system to identify factor which control cell proliferation-differentiation in vivo and study their mechanisms of action will be valuable to understand how the disruption of such regulatory processes results in pathological conditions such as cancers and may provide insights into therapeutic strategies.