CMFL AND MURINE CARDIAC DEVELOPMENT

研究建议：科学价值：这项工作的目的是建立 CMF-1的小鼠同源物的功能，CMF-1是一种新的蛋白， 被巴德实验室认为可能在鸡的心脏发生中起作用。 克隆小鼠同源基因的目的是最终敲除 在体外和体内建立表型。 而 目前该项目的科学价值是好的，初步的 数据是如此稀缺，淘汰性方法无法产生一个突出的 热情的程度。 在考虑击倒之前，一项特定的调查 应该迅速产生并用于整个安装和 面向截面的原位。 在生成这些数据之前， 进行三年的实验计划，实在是太弱了。

RESEARCH PROPOSAL: Scientific Merit: The aim of this work is to establish the function of the mouse homologue of CMF-1, a novel protein characterized by the Bader laboratory as possibly playing a role in chick cardiogenesis. The cloning of the mouse homologue will be aimed at eventually knocking it out and establishing the phenotype, both in vitro and in vivo. While the scientific merit of the project as it stands now is good, the preliminary data are so scarce that a knockout approach fails to engender an outstanding level of enthusiasm. Before contemplating a knockout, a specific probe should have rapidly been generated and used for both whole mount and section-oriented in situ's. Until these data are generated, the rationale for undertaking a three year experimental plan is simply too weak.