DIAGNOSIS AND CONTROL OF SIMIAN VARICELLA EPIZOOTICS

描述（改编自申请人摘要）：SVV引起高度 非人类灵长类动物的一种传染性的、威胁生命的疾病。 这种自然 旧大陆猴的疾病的特征是发热，水泡性皮疹， 并且经常传播疾病，包括肝炎和肺炎。 一旦 一旦开始，这种疾病可能会迅速蔓延到整个灵长类动物群体， 易感的猴子 几种主要的猿类水痘流行病 在过去的三十年里，在灵长类动物设施中偶尔发生。 在圈养的非人类种群中，这些流行病涉及高 发病率和有时高死亡率，导致研究资源的损失 有价值的研究数据。 适当的抗病毒治疗以控制 如果疾病被迅速诊断出来，就有可能发生动物流行病。 不幸的是， 快速诊断猴水痘的诊断方法 目前可用。 该应用程序的总体目标是开发 快速和特异性的诊断测定，这将有助于控制 灵长类动物设施中的SVV流行病。 免疫组织化学检测将 设计用于检测可接近组织中的特异性SVV抗原， SVV感染的猴子 利用聚合酶链反应（PCR）的试验 将开发用于检测受感染猴组织中的SVV核酸。 此外，还将开发酶联免疫测定法（ELISA）， 检测感染猴血清中SVV抗原抗体， 评价SVV暴露和猴水痘易感性。 本试验将用于猴的流行病学调查 在TRPRC的水痘。

DESCRIPTION (Adapted from the applicant's abstract): SVV causes a highly contagious, life-threatening disease in nonhuman primates. This natural disease of Old World monkeys is characterized by fever, vesicular skin rash, and often disseminated disease, including hepatitis and pneumonia. Once initiated, the disease may rapidly spread throughout a primate colony to susceptible monkeys. Several major simian varicella epizootics have occurred sporadically in primate facilities over the past three decades. These epizootics in captive nonhuman populations have involved high morbidity and sometimes high mortality, resulting in lost research resources and valuable research data. Appropriate antiviral therapy to control epizootics is possible if the disease is quickly diagnosed. Unfortunately, diagnostic methods for the rapid diagnosis of simian varicella are not currently available. The overall goal of this application is to develop rapid and specific diagnostic assays which will be useful for the control of SVV epizootics in primate facilities. Immunohistochemical assays will be designed to detect specific SVV antigens in accessible tissues in SVV-infected monkeys. Assays utilizing the polymerase chain reaction (PCR) will be developed to detect SVV nucleic acid in tissues of infected monkeys. In addition, an enzyme-linked immunoassay (ELISA) will be developed to detect antibodies to SVV antigens in sera derived from infected monkeys in order to evaluate exposure to SVV and susceptibility to simian varicella. This assay will be used to conduct an epidemiological survey of simian varicella at the TRPRC.