RAPID SCREENING FOR PL-A1 ALLOANTIGEN USING SOLID PHASE MICROPLATE IMMUNOASSAY

富含血小板的血浆使用Capture-P试剂盒(Immoor)进行检测。 微滴定井中的血小板单层与抗PL-1孵育。 A1，洗涤，暴露于抗免疫球蛋白G包被的红血中 细胞。包被的红细胞与PL-A1阳性的血小板黏附 但对PL-A1阴性的血小板不起作用。离心式沉淀法 未附着的红细胞会产生一个中心按钮，表明 血小板是PL-A1阴性。在520名捐赠者中，15人(2.88%)接受了测试 PL-A1阴性，这与报道的 文献中高加索人的PL-A2。所有15名PL-A1阴性供者 经等位基因特异性限制性内切酶分析证实。确认性 对于PL-A1阳性的献血者，没有进行基因分型。这个 PL-A1阴性献血者的比率与已发表的 高加索人的税率。这种化验方法可以用来筛查大量的人 样本数量。验证性测试可保留用于样本测试 PL-A1固相为负值。正在进行的工作包括确定 “假阳性”的比率。

Platelet rich plasmas are tested using the Capture-P kit (Immucor). Platelet monolayers in microtiter wells are incubated with anti-PL- A1, washed, and exposed to anti-immunoglobulin G-coated red blood cells. The coated red blood cells adhere to PL-A1 positive platelets but not to PL-A1 negative platelets. Centrifugal sedimentation of unattached red blood cells produces a central button, indicating that platelets are PL-A1 negative. Of 520 donors, 15 (2.88%) tested PL-A1 negative, which correlates well with the reported frequency of PL-A2 in caucasians in the literature. All 15 PL-A1 negative donors were confirmed by allele-specific restriction assays. Confirmatory genotyping was not done on those donors testing PL-A1 positive. The rate of PL-A1 negative donors correlates well with the published rate for caucasians. The assay can be used to screen large numbers of samples. Confirmatory testing can be reserved for sample testing PL-A1 negative by solid phase. Ongoing work involves identifying the rate of "false positives."