MODULATION OF PROTEIN KINASE ACTIVITY BY GENISTEIN IN PROSTATE CANCER

染料木素是一种异黄酮类化合物，在大豆中含量很高。它的 消费与前列腺癌的低发病率有关。 (PCA)。该项目试图阐明潜在的机制，通过 金雀异黄素可能对前列腺癌有潜在的治疗作用。 我们的研究阐明了一种潜在的抗肿瘤作用 金雀异黄素可增加PCa细胞对固体基质的黏附。 粘附力的增加表现出时间和浓度的依赖性， 黏附相关效应在染料木素低至 1 NM(含膳食消费，平均血清游离浓度 染料木素为10 NM)。 与细胞形态变化相关的是焦点移位 从细胞质到细胞膜的黏附激酶(FAK；一种酪氨酸酶) 相关的灶性黏附斑块(细胞黏附到的灶性区域 细胞外固体基质)。在膜上，FAK形成了一个与 β-1整合素(一种重要的跨膜细胞黏附分子)。这个 FAK自体激酶活性在转归过程中短暂升高 细胞黏附过程，此后降至基线以下 级别。

Genistein is an isoflavinoid which is found in high amounts in soy. Its consumption has been associated with a low incidence of prostate cancer (PCa). This project sought to elucidate potential mechanisms by which genistein may have potential therapeutic effects upon prostate cancer. Our studies elucidated a potential antimetastatic effect by demonstrating that genistein increased the adhesion of PCa cells to solid matrix. Increased adhesion was shown to be time and concentration dependent, with adhesion related effects evident at genistein concentrations as low as 1 nM (with dietary consumption, average serum concentrations of free genistein are 10 nM). Associated with changes in cell morphology was the translocation of focal adhesion kinase (FAK; a tyrosine kinase) from the cytoplasm to membrane associated focal adhesion plaques (focal areas of cell adhesion to extracellular solid matrix). At the membrane, FAK formed a complex with beta-1-integrin (an important transmembrane cell adhesion molecule). The activity of FAK autokinase activity transiently increased during the process of cell adhesion, thereafter it decreased to below baseline levels.