DNA SYNTHESIS AND RECOMBINATION BY HIV DNA POLYMERASE

描述：（改编自研究者摘要）：应用程序 将研究HIV-1进行链转移的机制。 具体的实验包括检查RNA酶H的作用， 转移过程中的病毒RT。 此外，RT在 促进转移反应和实验的影响 条件和病毒核衣壳蛋白的反应将是 测定 研究人员将确定突变的频率 伴随着同源与非同源重组。 突变 与转移反应相关的将与特定的 供体模板上的序列特征。 链转移检查 在奈韦拉平存在下的效率和诱变将决定 这种药物是否改变了链转移的效率， 诱变 耐药突变体RT将用于分析 链转移参数和诱变。 这些结果将表明 药物治疗或药物诱导的RT突变是否可以促进或 抑制病毒重组。

DESCRIPTION: (Adapted from investigator's abstract): The application will examine the mechanism of strand transfer carried out by HIV-1. Specific experiments include examination of the role of RNAse H of the viral RT in the process of transfer. Additionally, the roles of RT in promoting a transfer reaction and the influence of experimental conditions and viral nucleocapsid protein on the reaction will be determined. The investigator will determine the frequency of mutations accompanying homologous versus nonhomologous recombination. Mutations associated with the transfer reaction will be correlated to specific sequence features on the donor template. Examination of strand transfer efficiency and mutagenesis in the presence of Nevirapine will determine whether that drug alters the efficiency of strand transfer in mutagenesis. Drug resistant mutant RT will be used for analysis of strand transfer parameters and mutagenesis. These results will indicate whether drug treatment or drug induced RT mutations can promote or suppress viral recombination.