ENTAMOEBA CYST FORMATION

拟议的实验将探讨内阿米巴对包囊的反应， 诱导条件在细胞超微结构、基因水平 表达和蛋白质合成。 了解要求， 包囊细胞的反应将提供有用的信息， 设计方法来中断寄生虫生命周期的这一阶段， 从而降低了体内寄生虫感染形式的数量， 人口。 E. invadens最初将被用作模型系统 大肠溶组织剂 将优化囊肿形成的条件， 从体外滋养体培养物中获得高产量的包囊。 电子 显微镜将用于检查超微结构的变化， 包囊形成。 新的蛋白质合成将通过代谢检查 标记的包囊培养物和抗血清， 产品. 将分离并使用在包囊化期间表达的基因 监测寄生虫包囊形成过程中的转录反应。 支持E.将寻求溶组织剂， 为了开发一个体外包囊形成系统的人类寄生虫。

The proposed experiments will explore the response of Entamoeba to cyst- inducing conditions at the levels of cellular ultrastructure, gene expression and protein synthesis. Knowledge of the requirements and responses of the encysting cell will provide information useful for designing approaches to interrupt this stage of the parasite life cycle, thereby lowering the number of infective forms of the parasite in the human population. E. invadens will initially be used as a model system for E. histolytica. Conditions of cyst formation will be optimized to obtain high yields of cysts from in vitro trophozoite cultures. Electron microscopy will be used to examine ultrastructural changes during encystation. Novel protein synthesis will be examined with metabolically labelled encysting cultures and antisera which recognize cyst-specific products. Genes expressed during encystation will be isolated and used to monitor the transcriptional response of parasites during encystment. Conditions which support encystment of E. histolytica will be sought, in order to develop an in vitro cyst-forming system for the human parasite.