CONTROL OF PREF 1 GENE DURING ADIPOCYTE DIFFERENTIATION

Pref-1在前脂肪细胞中高度表达，但其转录 在脂肪细胞分化过程中减少，并且在脂肪细胞分化过程中未检测到IL-1。 在成熟的脂肪细胞中。事实上，当宪法表达， 在转染后，EST-I阻断3 T3-L1细胞的脂肪转化。的 下调β 1的机制尚不清楚。长期 这项提案的目的是了解β 1是如何下调的， 在脂肪形成过程中。拟议工作的具体目标是：（1） 鉴定β-半乳糖苷酶5 '侧翼区的顺式作用序列， 1基因，介导其转录下调，在脂肪细胞 （2）鉴定5 '-侧翼区的顺式作用序列 介导转录抑制作用的BMP 1基因的区域 地塞米松和15-脱氧-D12，14-前列腺素J2在3 T3-L1中 成纤维细胞;. (3)为了表征与细胞结合的反式作用因子， 目标1和2中确定的序列。缺失分析 启动子将定位参与抑制的顺式作用区域， 是1。生化绘图技术将揭示特定的DNA 调节蛋白结合的序列。

Pref-1 is highly expressed in presdipocytes, but its transcription decreases during adipocyte differentiation and pref-1 is not detectable in mature adipocytes. In fact, when constitutively expressed by transfection, pref-1 blocks adipose conversion of 3T3-L1 cells. The mechanism for the downregulation of pref-1 is unknown. The long term goal of this proposal is to understand how pref-1 is down-regulated during adipogenesis. The specific aims of the proposed work are: (1) To identify cis-acting sequences within the 5'-flanking region of the pref- 1 gene that mediate its transcriptional down-regulation during adipocyte conversion; (2) To identify cis-acting sequences within the 5'-flanking region of the pref-1 gene that mediate its transcriptional repression by dexamethasone and 15-deoxy-D12,14-prostaglandin J2 in 3T3-L1 fibroblasts;.(3) To characterize the trans-acting factors which bind to the sequences identified in aims 1 and 2. Deletion analysis of the promoter will locate the cis-acting regions involved in the suppression of pref-1. Biochemical mapping techniques will reveal the specific DNA sequences to which regulatory proteins bind.