TGF-B SUPERFAMILY AND MOUSE DEVELOPMENT

描述：（改编自申请人摘要）生长和分化 因子3/Vg-1相关蛋白（GDF-3/Vgr-2）、激活素BA和激活素BB是 转化生长因子β超家族成员。 议员 超家族在多种发育过程中起着重要作用。 为了帮助表征来自该蛋白质的蛋白质， 超家族，候选人打算研究GDF-3/Vgr-2的功能， 激活素使用以下策略： 本建议第一阶段的目标是：（1）进一步确定 GDF-3/Vgr-2基因通过研究该基因的转录模式， 并确定该基因在物种间的保守性;（2）分析 GDF-3/Vgr-2 mRNA在小鼠胚胎发育中的表达 用原位杂交技术检测成年小鼠组织中DNA的表达; GDF-3/Vgr-2基因在胚胎干细胞中的同源重组。 （四） 用激活素BB取代激活素BA成熟区，以确定是否 这些蛋白质在功能上可互换。 在第二阶段， 建议的目的是：（1）继续表征形态学， 所产生的突变小鼠的组织学和生化特征 在阶段1期间;和（2）杂交携带突变的小鼠， GDF-3/Vgr-2基因座与其他骨形态发生蛋白缺陷小鼠 和激活素。

DESCRIPTION: (Adapted from applicant's abstract) Growth and differentiation factor 3/Vg-1-related protein (GDF-3/Vgr-2), activin BA and activin BB are members of the transforming growth factor beta superfamily. Members of this superfamily play important roles in a variety of developmental processes. In an effort to aid in the characterization of proteins from this superfamily, the candidate intends to study the functions of GDF-3/Vgr-2 and the activins using the following strategies: In phase I of this proposal the aims are: (1) To further characterize the GDF-3/Vgr-2 gene by studying the pattern of transcription from this gene, and to determine the conservation of this gene among species; (2) To analyze the expression pattern of GDF-3/Vgr-2 mRNA in the developing mouse embryo and in adult mouse tissues by in situ hybridization; (3) To inactivate the GDF-3/Vgr-2 gene by homologous recombination in embryonic stem cells. (4) To substitute the activin BA mature region with activin BB to determine if these proteins are functionally interchangeable. In phase II of this proposal the aims are: (1) To continue to characterize the morphologic, histologic and biochemical characteristics of the mutant mice produced during phase 1; and (2) To crossbreed mice carrying a mutation in the GDF-3/Vgr-2 locus with other mice deficient in bone morphogenetic proteins and activins.