ORGANIZATION OF INFEROTEMPORAL CORTEX

描述：(摘自调查员的摘要)的长期目标 本研究旨在阐明被试在被试中的客体知觉机制。 灵长类视觉皮质。在目前的提案中，解剖学和 生理学技术将被用来分析模块化组织 并测定猕猴大脑皮质V2和V4区的 这些模块与V1区和次临时性的联系 大脑皮层。第一个目标是确定特定的模式 单个V2细胞色素氧化酶(CO)细条与 带V4区的条带间隔间。本征的光学记录 在活体内，皮质信号将被用于识别V2隔室。V2 细条纹将通过iso-1的相对激活来识别。 发光色的刺激物，而条纹间和粗条则 由它们的取向选择性和差异选择性来识别。 针对相邻细条和条带间的示踪剂注入 V2的舱室将比较它们在V4和V4中的终止模式 将确认他们来自V1的输入的性质。注射用药 可区分的示踪剂分为相同的功能不同的部分 条带将显示它们与V1和V4中的模块的特定连接。 细条纹中的功能异质性可能是由于分离 来自亮度的颜色信息或诸如 作为质地。对这些子模块的注入将测试这些 在V4中，功能上不同的细胞团投射到分离的病灶。 在互补性实验中，向不同方向的注射- V2条带间中的特定群集将测试V2是否投影到V4 以特定于方向的方式。第二个目标是确定 V4的功能体系结构，并确定 V4与V2和下颞叶皮质中功能定义的簇 (It)。首先，将使用光学记录来识别V2 隔间，在活体内。然后，将组合V4的光学记录 通过对单个V2细条和条间的微刺激 确定区域V4中这些V2隔间的终端域。 第二，V4中的光学记录将与视觉刺激相结合 识别优先激活的细胞团 颜色、亮度、方向、动态纹理或运动刺激。 可辨别的顺行和逆行示踪剂将被注入 这些V4车厢用来识别它们的终端在 V2和颞下皮质。这些实验测试了V4是否 分析对象边界的模块或分析表面的模块 颜色、亮度和质地的属性，投影到分离的位置 在IT行业。第三个目标是确定内在的组织 区域V2和V4内的电路。同样可辨别的顺序词 用于研究V2和V4外在联系的示踪剂将 揭示与细胞色素相关的局部回路 V2的氧化酶图谱和V2和V4的功能图谱。这些 实验测试了隔室之间的“串扰”程度 可能为产生更复杂的接受性提供了基础 这些区域中的字段属性。总体而言，这些实验将 提供对集成对象感知机制的新见解 在视觉皮质中。

DESCRIPTION: (from the investigator's abstract) The long term goal of this research is to elucidate the mechanisms of object perception in the primate visual cortex. In the current proposal, anatomical and physiological techniques will be used to analyze the modular organization of cortical areas V2 and V4 in macaque monkeys and to determine the connections of these modules with area V1 and with inferotemporal cortex. The first goal is to determine the specific patterns of connections of individual V2 cytochrome oxidase (CO) thin stripe and interstripe compartments with area V4. Optical recording of intrinsic cortical signals will be used to identify V2 compartments, in vivo. V2 thin stripes will be identified by their relative activation by iso- luminant color stimuli, while inter-stripes and thick-stripes will be identified by their orientation selectivity and disparity selectivity. Tracer injections targeted at adjacent thin and inter-stripe compartments of V2 will compare their patterns of terminations in V4 and will confirm the nature of their inputs from V1. Injections of distinguishable tracers into functionally distinct portions of the same stripe will reveal their specific connections with modules in V1 and V4. Functional heterogeneity in thin stripes may be due to the segregation of color information from brightness or other surface properties such as texture. Injections into these submodules will test whether these functionally distinct cell clusters project to segregated foci in V4. In complementary experiments, injections into separate orientation- specific clusters in V2 interstripes will test whether V2 projects to V4 in an orientation-specific manner. The second goal is to determine the functional architecture of V4 and to determine the connections of functionally defined clusters in V4 with V2 and inferotemporal cortex (IT). First, optical recording will be used to identify V2 compartments, in vivo. Then, optical recording of V4 will be combined with microstimulation of individual V2 thin stripe and interstripes to identify the terminal domains of these V2 compartments in area V4. Second, optical recording in V4 will be combined with visual stimulation to identify cell clusters that are preferentially activated by chromatic, luminance, orientation, dynamic texture, or motion stimuli. Distinguishable anterograde and retrograde tracers will be injected into these V4 compartments to identify the distribution of their terminals in V2 and inferotemporal cortex. These experiments test whether the V4 modules that analyze object borders or those that analyze the surface properties of color, brightness and texture, project to segregated loci in IT. The third goal is to determine he organization of the intrinsic circuits within areas V2 and V4. The same distinguishable anterograde tracers used in the study of the extrinsic connections of V2 and V4 will reveal the local circuits that will be correlated with the cytochrome oxidase pattern in V2 and the functional maps in V2 and V4. These experiments test the degree of "cross-talk" between compartments that might provide the basis for the generation of more complex receptive field properties in these areas. Overall, these experiments will provide new insights into the mechanisms of integrated object perception in the visual cortex.