RETROGRADE TRANSPORT/NUCLEAR IMPORT PATHWAY IN NEURONS

描述：中国大陆的再生和逆行运输的研究 海兔神经元鉴定出一组逆行运输的蛋白质 其中含有核进口信号。该信号肽序列 与核进口序列同源，存在于各种 其他蛋白质，如S40大T抗原和外源 带有该序列的蛋白质既可以逆行，也可以逆行 运输和核进口被引入轴突。蛋白质 包含这样的序列，现已在 老鼠。这些蛋白质被认为代表了 难以捉摸的神经损伤信号，被认为是细胞体的中介 神经损伤后的反应。此应用程序中的实验 旨在表征包含这些的内源蛋白质 序列及其相关多肽。它们的生物学 海兔和大鼠神经中的蛋白质将被探索，希望 提供对神经损伤后细胞体反应的洞察和 确定潜在的分子机制。

DESCRIPTION: Studies on regeneration and retrograde transport in Aplysia neurons identified a set of retrogradely transported proteins which contain a nuclear import signal. This signal peptide sequence is homologous to the nuclear import sequences found in a variety of other proteins, such as the S40 large T-antigen and exogenous proteins with this sequence attached are subject to both retrograde transport and nuclear import when introduced into the axon. Proteins containing such sequences have now been obtained and identified in rat. These proteins are thought to represent prototypes for the elusive nerve injury signal which is thought to mediate the cell body response following nerve injury. The experiments in this application are intended to characterize endogenous proteins containing these sequences and their associated polypeptides.The biology of these proteins in Aplysia and rat nerve will be explored in the hopes of providing insights into the cell body response after nerve injury and identifying the underlying molecular mechanisms.