INTERACTIVE LIVE CELL AND TISSUE ANALYSIS SYSTEM

我们的主要目标是在广泛的领域内提高活细胞和组织的研发水平。 通过设计、建造和销售一种高性能的 快速交互式活细胞和组织成像显微镜系统， 用户友好的专用应用软件。 成像活细胞科学和生物技术的发展创造了一个 重要控制和信息处理问题， 通过技术的组合解决：快速，高分辨率的数字 冷却CCD相机，光学高效荧光显微镜， 特定应用程序的自动化，以及一台多处理器台式计算机 运行高效灵活的核心软件， 采集、处理、分析和显示。 我们第一阶段的具体目标是l）建立和测试一个原型基础 系统，2）扩展原型与非侵入性三维血管内皮细胞监测 模式，3）证明系统作为功能活细胞研究工具，4） 详细说明第二阶段的完整系统，5）编写第一阶段报告。 拟议的商业应用： 最终产品将用于促进和扩大分析， 活细胞和组织，并将用于临床和研究 设置.例如：发育生物学，细胞生物学， 毒理学药物研发该系统还将具有重要的 在组织工程等领域的临床和研究应用， 或移植技术。最终产品将对研究人员有用 在大学，生物技术实验室，政府设施， to clinicians临床医生at hospital医院.

Our main objective is to improve live cell and tissue R&D over a broad range of applications by designing, constructing, and marketing a high speed, interactive, live cell and tissue imaging microscope system with dedicated user-friendly application software. The growth in imaging live cell science and biotechnology has created a significant control and information processing problem that can now be solved by a combination of technologies: a fast, high-resolution digital cooled CCD camera, an optically efficient fluorescence microscope with application-specific automation, and a multiprocessor desktop computer running highly efficient and flexible core software for control, image acquisition, processing, analysis and display. Our specific aims for phase I are to l) build and test a prototype basic system, 2) expand the prototype with a non-invasive 3D VEC monitoring mode, 3) demonstrate the system as a functional live cell study tool, 4) specify the complete system for phase II, 5) write the phase I report. PROPOSED COMMERCIAL APPLICATIONS: The end product will be used to facilitate and expand the analysis of living cells and tissues and will be used in both clinical and research settings. Examples include: developmental biology, cell biology, toxicology, drug development. The system will also have important clinical and research applications in areas such as tissue engineering, or transplant technology. The end product would be useful to researchers at universities, biotechnology labs, Government facilities and eventually to clinicians at hospital.