TITRIMETRIC DETERMINATION OF HEPARIN IN WHOLE BLOOD

这个项目的目标是开发一种简单、定量的 肝素的护理点检测，一种用于防止凝血的药物 在大多数外科手术中，尤其是那些需要 体外循环。随着游离肝素的持续存在， 手术对病人是危险的，通常要用鱼精蛋白 逆转肝素的抗凝作用 做手术。肝素测定最常用的方法是一种非 测量病人激活的凝血时间的特定生物测定法 血。第一阶段的研究将证明测量的可行性。 一次性鱼精蛋白滴定法测定全血中的肝素 弹药筒。滴定终点由光学元件确定 鱼精蛋白特异的发色团的反射光谱测量 加入到一种亲脂聚合物中，浇注在高度反射的 薄膜。肝素用几个含有不同成分的试剂盒进行滴定 与样品的游离肝素形成络合物的鱼精蛋白的量，以及 然后测量是否存在过多的鱼精蛋白。在第二阶段，我们 将构建一个原型仪器，它可以自动执行 全血中肝素的鱼精蛋白滴定。 建议的商业应用 实用的护理点仪器，可以直接监控 患者的全血样本中的肝素水平将非常高 对肝素化患者的管理很有用。与之相比 目前的生物检测，所提出的光学传感器提供了更好的 专一性，从而消除了很大程度上的不确定性 评估患者出血的原因，从而导致过度- 鱼精蛋白的处方。

The objective of this project is to develop a simple, quantitative point-of-care test for heparin, a drug that is used to prevent clotting during most surgical procedures, especially those requiring an extracorporeal loop. As the continued presence of free heparin after surgery is dangerous to the patient, protamine is usually administered to reverse the anticoagulant effect of heparin at the conclusion of surgery. The method most often used for heparin measurement is a non- specific bioassay that measures activated clotting time of a patient's blood. Phase I studies will demonstrate the feasibility of measuring heparin in whole blood samples using protamine titration in a disposable cartridge. The titration end-point is determined by an optical reflectance measurement of a protamine-specific chromoionophore incorporated into a lipophilic polymer cast onto a highly reflective membrane. Heparin is titrated with several cartridges containing varying amounts of protamine which complexes the free heparin of the sample, and the presence of any excess protamine is then measured. In Phase II we will construct a prototype instrument that can automatically perform a protamine titration of heparin in whole blood samples. PROPOSED COMMERCIAL APPLICATION Practical point-of-care instrumentation that could directly monitor heparin levels in whole blood samples from patients would be extremely useful in the management of heparinized patients. Compared to the current bioassay, the proposed optical sensor offers much better specificity, thereby eliminating a large degree of uncertainty in assessing the cause of patient bleeding and consequently the over- prescription of protamine.