DEVELOPMENT OF OPTICAL DEVICE FOR USE IN DRUG DISCOVERY

描述（研究者摘要）：本提案涉及 NINDS的分子神经生物学兴趣来自最新的Omnibus 教唆 一种高通量、基于荧光的技术， 测量受体介导的细胞反应。 目标是 开发一种能够测量细胞内Ca 2+的光学探针 由荧光染料报告的用于高容量应用的信号， 筛选一些合成或天然化合物。 一些 报道染料现在可用于测量细胞内Ca 2+信号 以下表面膜受体活化和方法存在测试 如果这些信号是通过变力性或代谢性机制转导的话。 光学探针设计将结合有效的光通量， 细胞灌注和快速数据采集。 商业应用 一个快速、廉价和定量的检测方法， 将成为可提供的作为合同服务的受体 生物制药公司，政府和大学， 在搜索中筛选新的合成和天然化合物 用于新型神经活性药物。 的主要优点 方法包括：1）独立于放射性同位素; 2）独立于 从实验室动物; 3）快速，简单和低成本; 4）高 体积通量; 5）受体占有率的定量测量， 有效性; 6）适应数百种受体和亚型。 拟议商业应用：不可用

DESCRIPTION (Investigator's Abstract): This proposal addresses the Molecular Neurobiology interest of NINDS from the latest Omnibus solicitation. A high throughput, fluorescence based technique to measure receptor mediated cellular responses is proposed. The goal is to develop an optical probe capable of measuring intracellular Ca2+ signals reported by fluorescent dyes for high volume application such as screening a number of synthetic or natural compounds. A number of reporter dyes are now available to measure intracellular Ca2+ signals following surface membrane receptor activation and methods exist to test if these signals are transduced by inotropic or metabotropic mechanisms. The optical probe design will incorporate efficient light throughput, cell perfusion and fast data acquisition. The commercial application of the development is that a rapid, inexpensive and quantitative assay will become available to be offered as a contract service by Receptor Biology to pharmaceutical companies, government and universities to screen novel synthetic and naturally occurring compounds in the search for novel neuro-active pharmaceuticals. The major advantage of the method include: 1) independence from radioisotopes; 2) independence from laboratory animals; 3) rapidity, simplicity and low cost; 4) high volume throughput; 5) quantitative measure of receptor occupancy and efficacy; 6) adaptable to hundreds of receptors and subtypes. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE