BETA-GLUCAN RECEPTOR

描述：严重创伤、伤口和败血症导致全身性 抑制宿主防御。 哺乳动物巨噬细胞最近拥有 描述了识别β-1，3聚合物的细胞表面受体， 葡萄糖 这种β-葡聚糖受体提供了一种调理素- 微生物的独立识别和清除。 结合 β-葡聚糖受体增强了 巨噬细胞 用于结合免疫球蛋白的Fc部分的受体和 补体受体是巨噬细胞良好表征标记物 activation. 已知这些调理素受体在以下情况下受损： 脓毒症动物的中性粒细胞。 β-葡聚糖受体的作用 目前是未知的，并且是本申请的焦点。 这些 研究将详细说明的结构和绑定特性的 使用标准方法对受体的数量和亲和力进行定量。 的 β-葡聚糖的表达将在静息和活化状态下确定。 来自不同解剖部位的细胞 实验将比较beta- 葡聚糖受体表达与调理素受体表达， 将激活状态和免疫功能的相似性和差异性 功能 这些研究将检验非调理素 β-葡聚糖受体在宿主防御中起着更大的作用， 调理素依赖性吞噬作用受损的条件。

DESCRIPTION: Severe trauma, wounds and sepsis result in generalized suppression of host defenses. Mammalian macrophages possess a recently described cell surface receptor that recognizes beta-1,3 polymers of glucose. This beta-glucan receptor provides a means for opsonin- independent recognition and clearance of microorganisms. Binding to beta-glucan receptors potentiates the phagocytic function of macrophages. Receptors for binding the Fc portion of immunoglobulin and the complement receptor are well characterized markers of macrophage activation. These opsonic receptors are known to be compromised in neutrophils from septic animals. The role of the beta-glucan receptor is presently unknown and is the focus of this application. These studies will detail the structure and binding characteristics of the receptor using standard methods to quantitate number and affinity. The expression of beta- glucan will be determined in resting and activated cells from various anatomic sites. Experiments will compare the beta- glucan receptor expression with the expression of opsonic receptors and correlate similarities and differences with activation state and immune function. These studies will test the hypothesis that the non-opsonic beta-glucan receptor plays a greater role in host defense under conditions of compromised opsonin-dependent phagocytosis.