COMPARTMENTAL ORGANIZATION OF THE YEAST GOLGI COMPLEX

描述（改编自申请人的摘要）：格雷厄姆博士的长期 目的是了解细胞如何组装和维持有序的 组成高尔基复合体的隔室的排列，以及如何 这种结构有助于这种基本细胞器的功能。 具体目标是：（1）继续界定 组织的酵母高尔基复合体和所需的因素， 蛋白质运输之间的高尔基室;（2）破译 酵母高尔基体的驻留蛋白内的定位信号 复合物，Mnn 1蛋白（Mnn 1 p）;（3）分离突变体， Mnn 1 p的高尔基体定位缺陷;以及（4）分离基因 编码酵母特异性分选信息的片段 分泌途径

DESCRIPTION (adapted from applicant's abstract): Dr. Graham's long term goal is to understand how the cell assembles and maintains the ordered arrangement of compartments that comprise the Golgi complex, and how this structure contributes to the function of this essential organelle. The specific aims are: (1) to continue to define the compartmental organization of the yeast Golgi complex and the factors required for protein transport between Golgi compartments; (2) to decipher the localization signal(s) within a resident protein of the yeast Golgi complex, the Mnn1 protein (Mnn1p); (3) to isolate mutants that exhibit a defect in the Golgi localization of the Mnn1p; and (4) to isolate gene fragments that encode sorting information specific to the yeast secretory pathway.