GENETIC MONITORING OF RODENTS

本合同的目的是提供遗传监测资源， 确保近交系种群（例如F344大鼠、B6 C3 F1）的遗传完整性 用于毒理学和其他研究的小鼠和特殊品系 以及NTP和NlEHS的测试程序。多达20个指定位点将 通过各种电泳来监测每种菌株或杂交种。 组织匀浆和血清蛋白。杂交种的亲本， 杂交种和自交系从基础、扩繁到生产 定期评价菌落的遗传完整性。 近交系啮齿动物的遗传完整性也将通过皮肤移植进行评价。 适当时，限制性片段长度多态性（RFLP）和 通过PCR的DNA的简单序列长度多态性（SSLP）可以 评估以识别遗传方差。测试的遗传完整性 动物对于开发可靠和准确的研究数据至关重要， 动物实验。持续监控基础和生产 提供用于研究的菌落和动物将确保来自 NTP和NIEHS动物研究将从遗传同质的 啮齿动物试验动物的遗传同质性将是至关重要的， 比较不同项目和方案的研究，作为背景 损伤，试验机构之间的损伤，化学或 物理试剂和在同一实验室加班。

The purpose of this contract is to provide genetic monitoring resource to assure the genetic integrity of inbred stock such as F344 rats, B6C3F1 mice and special strains produced for the toxicology and other research and testing programs of the NTP and NlEHS. Up to 20 designated loci will be monitored for each strain or hybrid by electrophoresis of various tissue homogenates and serum proteins. Parental strains of hybrids, hybrids and inbred strains from the foundation, expansion and production colonies will be evaluated for genetic integrity at periodic intervals. Genetic integrity of inbred rodents will be evaluated by skin grafts also. When appropriate, restriction fragment length polymorphisms (RFLPs) and simple sequence length polymorphisms (SSLPs) of DNA by PCR may be evaluated to identify genetic variance. The genetic integrity of the test animals is essential for developing reliable and accurate research data in animal experiments. Continuous monitoring of foundation and production colonies and animals supplied for studies will ensure that data from the NTP and NIEHS animal studies will be collected from genetically homogenous rodents. Genetic homogeneity of test animals will be essential for comparison of studies from different projects and programs for background lesions, lesions between testing facilities, lesions between chemical or physical agents and within the same laboratory overtime.