LYMPHOCYTIC GABAA RECEPTORS

描述（申请人的摘要）：T细胞表达针对T细胞的受体。 内源性神经活性分子，如阿片样物质，P物质，和 生长抑素，其可以调节T细胞反应。 γ-氨基丁酸 γ-氨基丁酸（GABA）是中枢神经系统中主要的抑制性神经递质 系统 研究人员的初步研究表明，T 细胞表达功能性GABA-A受体。 激活GABA-A 受体抑制T细胞增殖和IL-2产生。 此外，委员会认为， GABA体内给药抑制了 胰岛素依赖性糖尿病（IDDM）至非肥胖糖尿病（NOD）小鼠。 这些初步发现表明，淋巴细胞GABA受体可以 下调炎症过程。 这项提案将考验 淋巴细胞GABA受体与神经元受体一样， 1）由在不同细胞上差异表达的亚基组成， T细胞群在发育过程中，并且2）它们的表达可以是 受多种因素调节，包括T细胞的活化、配体 GABA-A受体细胞因子和胰岛素 申请人还将测试 淋巴细胞GABA-A受体的激活可以调节 T细胞功能，包括其细胞因子和表面分子表达， 和细胞毒性。 申请人将描述以下机制： GABA介导其对T细胞的免疫调节作用。 最后，由于GABA 抑制体外T细胞增殖和过继转移 在体内糖尿病，他们假设GABA将抑制抗原特异性 迟发型超敏反应（DTH），以及自发性 NOD小鼠体内糖尿病的发展。 证明GABA 抑制Th 1介导的体内炎症反应可能导致新的 抑制T细胞介导的炎症过程的方法， 自身免疫性疾病 因此，这项提案的结果将提供基本的 深入了解T细胞的神经免疫调节的新模式， 炎症反应。 此外，该结果可能具有潜在的临床意义。 用于下调人的炎症过程。

DESCRIPTION (Applicant's Abstract): T cells express receptors for endogenous neuroactive molecules such as opioids, substance P, and somatostatin which can modulate T cell responses. Gamma-amino butyric acid (GABA) is the major inhibitory neuro-transmitter in the central nervous system. The investigators' preliminary studies have demonstrated that T cells express functional GABA-A receptors. Activation of these GABA-A receptors inhibited T cell proliferation and IL-2 production. Moreover, administration of GABA in vivo inhibited the adoptive transfer of insulin-dependent diabetes mellitus (IDDM) to non obese diabetic (NOD) mice. These preliminary findings suggest that lymphocytic GABA receptors can down-regulate inflammatory processes. This proposal will test the hypothesis that like their neuronal counterparts, lymphocytic GABA receptors are 1) comprised of subunits which are differentially expressed on different T cell populations during development, and that 2) their expression can be regulated by several factors including the activation of T cells, ligands for GABA-A receptors, cytokines, and insulin. The applicant will also test the hypothesis that activation of lymphocytic GABA-A receptors can modulate T cell function, including their cytokine and surface molecule expression, and cytotoxicity. The applicant will characterize the mechanism(s) by which GABA mediated its immuno-modulatory action on T cells. Finally, since GABA inhibited T cell proliferation in vitro and the adoptive transfer of diabetes in vivo , they hypothesize that GABA will inhibit antigen-specific delayed type hypersensitivity (DTH) responses, as well as the spontaneous development of diabetes in NOD mice in vivo. Demonstrating that GABA inhibits Th1-mediated inflammatory responses in vivo could lead to novel approaches for inhibiting T cell-mediated inflammatory process and autoimmune disease. Thus, the results of this proposal will provide basic insights into a new mode of neuro-immunological regulation of T-cells and inflammatory responses. Moreover, the results may have potential clinical application for down-regulating inflammatory processes in man.