CONTROL OF HYPOTHALAMIC TYROSINE HYDROXYLASE

描述（改编自研究者摘要）：主要目标 本申请的目的是评价细胞内机制， PRL、E和P影响TIDA神经元TH活性。 目标1将解决 TH的磷酸化的潜在作用， PRL升高 影响TH作为对PRL的反应的蛋白激酶将 被研究。 第二个目标是研究E 改变胎儿下丘脑细胞培养系统中的TH活性。 的 研究将确定E是否改变TH mRNA水平。 第三 目的将集中在P对TH活性的急性抑制作用， 胎儿下丘脑培养细胞，并将解决的问题， P是否能诱导TH去磷酸化。

DESCRIPTION (Adapted from the Investigator's Abstract): The major goals of this application are to evaluate intracellular mechanisms which permit PRL, E, and P to affect TH activity in TIDA neurons. Aim 1 will address the potential role of phosphorylation of TH during conditions of elevated PRL. Protein kinases which affect TH as a response to PRL will be studied. The second aim will examine the mechanisms by which E alters TH activity in a fetal hypothalamic cell culture system. The investigations will determine whether E alters TH mRNA levels. The third aim will focus on acute inhibitory effects of P on TH activity in the fetal hypothalamic cultured cells, and will address the question of whether P can induce dephosphorylation of TH.