GENETIC REGULATION OF THE HEPATIC ACUTE PHASE RESPONSE

描述：组织损伤和感染引发局部炎症 这一反应旨在动员 构成急性期(AP)反应和先天免疫的生物体 回应。诱导肝脏急性期血浆蛋白的产生 由所有炎症过程引起的系统效应之一。 结合珠蛋白(Hp)是血浆中的原血红蛋白结合蛋白。 哺乳动物的主要应用程序之一。惠普协调以下功能 炎性细胞，并有助于抑制细菌的环境。这个 该项目的目标是在小鼠模型中确定AP诱导的幽门螺杆菌的作用 控制炎症反应。具体目标是：1)识别 幽门螺杆菌基因在细胞培养中的诱导机制。三种类型的 所有的实验都遵循标准程序，使用 瞬时转染人肝癌细胞系HepG2细胞。在第一个中，Sequence元素 与HP启动子115个碱基区域结合的因子将进一步 被解剖了。在第二个中，外显子在STAT1和STAT3之间的改组是 建议在本系统中定义后者的功能域。在 第三，提出了在近端外部进行序列元素识别 启动子区域。2)建立幽门螺杆菌缺陷小鼠模型。自交系和同源幽门螺杆菌 将建立空小鼠。Hp+/-和Hp-/-动物对 具有代表性的炎症挑战，包括组织损伤、内毒素 感染将由急性时相蛋白基因的模式来定义 在肝脏中诱导和急性期蛋白组成的变化 血液中的白细胞。3)验证惠普在这些方面的具体行动 零动物和细胞培养系统。响应模式的差异可能 提示幽门螺杆菌的潜在功能，将在幽门螺杆菌空白动物身上进行验证 通过Hp给药或腺病毒重新引入肝脏Hp的产生。 幽门螺杆菌在分化功能中的细胞作用 炎性细胞将在组织培养系统中确定。4)至 确定删除Hp急性期诱导的后果 表情。幽门螺杆菌启动子中IL-6反应元件缺失的小鼠 由同源重组和靶向突变产生 接受急性期刺激，以评估这些因素的贡献 序列对幽门螺杆菌基因调控。

DESCRIPTION: Tissue damage and infection initiate a local inflammatory reaction that is designed to mobilize the immediate defense mechanisms of the organism, constituting the acute phase (AP) response and innate immune responses. Induction of hepatic acute phase plasma protein production is one of the systemic effects elicited by all inflammatory processes. Haptoglobin (Hp), the pronciple hemoglobin-binding protein in the plasma is one of the major APPs in mammals. Hp moderates the functions of inflammatory cells, and contributes to a bacteriostatic milieu. The project's goal is to define, in the mouse model, the roles of AP induced Hp controlling inflammatory responses. The specific aims are: 1) To identify the mechanism of Hp gene induction in cell culture. Three types of experiments are proposed all of which follow standard procedures employing transient transfection into HepG2 cells. In the first, sequence elements and factors which bind to a 115 bp region of the Hp promoter will be further dissected. In the second, exon shuffling between Stat1 and Stat3 is proposed to define functional domains of the latter in this system. In the third, sequence element identification is proposed outside the proximal promoter region. 2) To establish Hp-deficient mice. Inbred and congenic Hp null mice will be established. The response of Hp +/- and Hp -/- animals to representative inflammatory challenges, including tissue damage, endotoxin and infections will be defined by the pattern of acute phase protein genes induced in the liver and changes in composition of acute phase proteins and leukocytes in the blood. 3) To verify the specific action of Hp in these null animals and cell culture systems. Differences in response pattern may suggest potential functions of Hp which will be verified in Hp null animals by Hp administration or adenovirally re-introduced hepatic Hp production. The cellular action of Hp on differentiated functions associated with inflammatory cells will be determined in tissue culture systems. 4) To determine the consequence of a deleted acute phase induction of Hp expression. Mice null of the IL-6 response element in the Hp promoter will be generated by homologous recombination and targeted mutatgenesis and subjected to acute phase stimulants to assess the contribution of these sequence to Hp gene regulation.