RETINAL GLUTAMATE IN EXPERIMENTAL GLAUCOMA

描述：（来自摘要）研究者打算探索 原发性开角型青光眼视网膜神经节细胞死亡的基础 青光眼 她的调查是基于以前的工作， 青光眼患者的玻璃体中谷氨酸盐含量异常高。 她打算首先探索玻璃体谷氨酸盐与 集中和昏迷性损害。 这将通过气体进行 色谱/质谱在猴子的眼睛配对， 单眼实验诱导的青光眼。 她打算把 谷氨酸升高的幅度与神经纤维损失的程度有关。 其次，她提出了一个假设， 谷氨酸是穆勒细胞中扰动的结果。 视网膜 已知Muller细胞在从细胞中去除谷氨酸中起主要作用。 细胞外空间，以及随后的代谢。 的假设 因此，提出玻璃体谷氨酸水平升高可能是 这是穆勒细胞活性改变的结果。 她打算带着 进行免疫细胞化学分析，以评估Muller细胞 谷氨酸代谢的活性。 希望基于这些发现， 将找到一个解释，为谷氨酸水平升高， 玻璃体，以及识别潜在的新的药理学试剂。

DESCRIPTION: (from the abstract) The investigator intends to explore the underlying basis of retinal ganglion cell death seen in primary open angle glaucoma. Her investigations are based on previous work that has suggested that glutamate is abnormally high in the vitreous of patients with glaucoma. She intends to explore first the relationship between vitreal glutamate concentration and glaucomatous damage. This will be carried out by gas chromatography/mass spectroscopy in the paired eyes of monkeys with monocular experimentally induced glaucoma. She intends to correlate the magnitude of the glutamate elevation with the degree of nerve fiber loss. Secondly, she presents the hypothesis that the elevation in vitreal glutamate is a consequence of a perturbation in Muller cells. Retinal Muller cells are known to play a major role in the removal of glutamate from the extracellular space, and in its subsequent metabolism. The hypothesis is therefore presented that the elevated vitreal glutamate level can be the result of alterations in the activity of Muller cells. She intends to carry out immunocytochemical analyses to assess alterations in Muller cell activity in glutamate metabolism. It is hoped, based on these findings, that an explanation will be found for the elevated level of glutamate in the vitreous, as well as identifying potential new pharmacologic agents.