ANALYSIS & PURIFICATION OF DNA IN MICROFABRICATED ARRAYS

这项提案的重点是开发微制造器件，以 分离和分析染色体大小的DNA分子。它将利用 在微电子行业获得的经验 硅基器件的微细加工。现在的设施和专业知识 存在于大规模地设计和制造 微观结构可用于分析、量化和 分子量大小不等的分子的分离 几千到几百万个碱基对。 对DNA电泳法的理解具有重要的理论和实践意义 实际的重要性。目前的方法仅限于分离， 在合理的时间范围内，分子不超过10-7个碱基 成对的。这其中的部分原因是可以理解的，但仍有许多原因。 既要有实践性，又要有理论性。这项建议 建议进行旨在加深我们对理论的理解的实验 在创造能够分离分子的小型设备的同时， 大小，以小时为单位，而不是以周为单位。因为这些设备有两个 维度，许多与研究聚合物相关的问题 三个维度被绕过了。因为它们可以被制造出来 充分利用技术优势，规模化廉价生产 建立在固态行业，它们是实用的，并不 产生大量的废化学品。

This proposal focuses on the development of microfabricated devices to separate and analyze chromosome-size DNA molecules. It will exploit the experience gained in the microelectronics industry for the microfabrication of Si-based devices. The facilities and expertise now exist for the design and manufacture, on a large scale, of microstructures that can be used for the analysis, quantification, and separation of molecules whose molecular weights range in size from a few thousand to many millions of base pairs. An understanding of DNA electrophoresis is of great theoretical and practical importance. Current methods are limited to the separation, in a reasonable time frame, of molecules no larger than 10-7 base pairs. The reasons for this are partially understood, but much remains to be done of both a practical and theoretical nature. This proposal suggests experiments designed to deepen our understanding of theory while creating small scale devices capable of separating molecules this size and larger in hours rather than weeks. Because the devices are two dimensional, many of the problems associated with studying polymers in three dimensions are circumvented. Because they can be manufactured cheaply on a large scale by taking advantage of techniques well established in the solid state industry, they are practical and do not create large quantities of spent chemicals.