PREGNANCY EFFECTS ON ENDOTHELIAL FUNCTION

描述(改编自《调查员摘要》)：调查员 建议解决怀孕会降低血管紧张度和 增强雌激素敏感的内皮依赖性的反应性 机械装置。具体地说，他们假设雌激素能诱导 降低血管张力的内皮酶和平滑肌酶 和反应性。因此，器官对雌二醇的敏感性发生了变化 最终决定了怀孕期间血流的重新分配。 研究人员建议研究怀孕和雌二醇对 通过cGMP或cGMP调节血管活性的酶 使用妊娠、非妊娠和雌二醇处理的去势豚鼠的cAMP 猪。将解决四个具体目标：1)确定 妊娠和雌二醇对元素的定位和转录的影响 对调节血管反应性至关重要；2)确定 孕期和雌二醇对孕期重要酶活性的影响 调节血管反应性；3)确定妊娠和 雌激素对G蛋白偶联血管反应的影响；以及4)确定 怀孕会改变阻力血管的内皮功能。

DESCRIPTION (Adapted from the Investigator's Abstract): The investigators propose to address the hypothesis that pregnancy decreases vascular tone and reactivity by enhancing estradiol-sensitive endothelium-dependent mechanisms. Specifically, they hypothesize that estradiol induces endothelial and smooth muscle enzymes whose actions decrease vascular tone and reactivity. Thus, variation in organ sensitivity to estradiol ultimately determines the redistribution of blood flow during pregnancy. The investigators propose to study the effect of pregnancy and estradiol on enzymes responsible for modulating vascular reactivity via either cGMP or cAMP using pregnant, nonpregnant, and estradiol-treated castrate guinea pigs. Four specific aims will be addressed: 1) determine the effect of pregnancy and estradiol on the location and transcription of elements critical for modulating vascular reactivity; 2) determine the effect of pregnancy and estradiol on the specific activity of enzymes important in modulating vascular reactivity; 3) determine the effect of pregnancy and estradiol on G-protein coupled vascular responses; and 4) determine whether pregnancy alters endothelial function in resistance vessels.