EVALUATION OF REPORTED EMF EFFECTS ON CELL COMMUNICATION

描述：拟议工作的主要目标是准确地 重复Benane等人报告的研究。(1996)，其中克隆9个细胞， 用部分抑制GAP的水合氯醛浓度处理 连接细胞间通信(GJIC)，暴露在电力中 频率，即极低频(ELF)，电磁场 (电磁场)。据报道，ELF-EMF产生的GJIC进一步减少 与作者提出的离子参数共振(IPR)模型一致 布莱克曼等人。(1993)，它预测了不同的磁场相互作用 与生物系统有关。所有相关的设备和专业知识 实验技术已经到位，可以执行这些复制 橡树岭国家实验室(ORNL)的研究。如果相同的结果是 使用与Blackman相同的曝光系统进行观察 同事，那么效果的一致性将在 不同曝光系统的限制和对 实验方案。专用磁场曝光系统位于 ORNL是美国四个地区性设施之一，将 用于重复和扩展复制研究。这套曝光系统 旨在为细胞培养提供精心控制的条件 暴露在磁场中。此外，还拥有丰富的 首席研究员实验室与GJIC的研究表明有可能 用于测量GJIC的实验技术的改进。 刮擦装载程序的改进和电生理学的应用 对这些后续的细胞间研究提出了测量方法 耦合。如果上面概述的主要目标证实了结果 布莱克曼和同事的报告，那么重点将转移到 旨在了解相关机制的实验。

DESCRIPTION: The major objective of the proposed work is to exactly replicate studies reported by Benane et al. (1996) in which Clone 9 cells, treated with concentrations of chloral hydrate which partially inhibit gap junctional intercellular communication (GJIC), are exposed to power frequency, i.e. extremely-low-frequency (ELF), electromagnetic fields (EMFs). A further reduction in GJIC produced by the ELF-EMF was reported to be consistent with the Ion Parametric Resonance (IPR) model, proposed by Blackman et al. (1993), which predicts distinct magnetic field interactions with biological systems. Equipment and an expertise in all relevant experimental techniques is already in place to perform these replication studies at Oak Ridge National Laboratory (ORNL). If the same results are observed using an identical exposure system as that used by Blackman and co-workers, then the consistency of the effect will be examined under the constraints of a different exposure system and modifications of the experimental protocol. The dedicated magnetic field exposure system at ORNL, which is one of four regional facilities in the United States, will be used to repeat, and expand the replication studies. This exposure system was designed to provide carefully controlled conditions for cell culture exposures to magnetic fields. In addition, extensive experience in the principal investigator's laboratory with GJIC studies suggests possible improvements in the experimental techniques used to measure GJIC. Modifications in scrape-loading procedures and the use of electrophysiology measurements are proposed for these subsequent studies of cell-to-cell coupling. If the main objectives, outlined above, substantiate the results reported by Blackman and co-workers then emphasis will be shifted to experiments aimed at understanding the mechanisms involved.