MOLECULAR CONTROL OF ICAM-1 EXPRESSION IN ORAL CANCER

描述：ICAM-1是一种细胞表面蛋白，参与 细胞与细胞之间的相互作用，包括免疫反应和癌症。 其 高风险人乳头瘤病毒上调表达， 首席研究员所在机构的研究人员已经建立了三个 不同转化水平的口腔细胞系， ICAM-1表达水平。 调查员现在将检查 ICAM-1表达在这些细胞中调节的机制。 ICAM-1 将通过以下方法确定每个细胞系中的mRNA积累和半衰期： 北方印迹。 反式激活因子的活性将通过 电泳迁移率变动分析。 希望能理解 ICAM-1在转化细胞中表达的调节可能导致 控制肿瘤生长的最佳策略。

DESCRIPTION: ICAM-1 is a cell-surface protein which is involved in cell-cell interactions, including in the immune response and in cancer. Its expression is upregulated by high-risk human papillomaviruses, and other investigators at the principal investigator's institution have set up three oral cell lines with different levels of transformation, and different levels of ICAM-1 expression. The investigator will now examine the mechanisms by which ICAM-1 expression is regulated in these cells. ICAM-1 mRNA accumulation and half life in each cell line will be determined by northern blots. Activities of transactivating factors will be looked for by electrophoretic mobility shift assays. It is hoped that an understanding of the modulation of ICAM-1 expression in transformed cells could lead to optimal strategies in controlling tumor growth.