DEVELOPMENTAL EFFECTS OF FISH BORNE TOXICANTS IN THE RAT

描述：（改编自研究者摘要）最近 流行病学研究表明， 孕妇污染五大湖鱼类， 他们的婴儿和儿童的认知缺陷。 虽然多氯 联苯（PCB）暴露与这些功能障碍相关，最近的数据显示， 我们收集了围产期暴露于饮食的大鼠， 冷冻五大湖鲑鱼的研究表明， 影响可能是由于鱼中的其他神经活性污染物或 多氯联苯和其他污染物之间的协同作用。 为了 确定五大湖鱼类中存在的哪些有毒物质会诱导神经行为 围产期暴露后的变化，我们将：（1）测量 安大略湖有机和无机污染物浓度 (LO)鲑鱼和太平洋（PO）鲑鱼，其将作为对照 在所有实验中;（2）确定大鼠的神经行为效应 围产期暴露于冻干LO鲑鱼（含有所有污染物）或 LO鲑鱼的硫酸提取物（含有所有有机污染物）。 如果冻干的LO鲑鱼和溶剂的神经行为效应 浸提物相等，我们将假设不可浸提组分 （包括甲基汞）不参与介导的影响，我们将继续 （3）测定两种弗罗里硅土柱的神经行为效应 LO溶剂提取物的级分-一个含有大部分的 多氯联苯、DDE、六氯代苯、灭蚁灵和氯丹，以及其他含有共平面 多氯联苯、二恶英、二苯并呋喃和极性农药;（4）测定 暴露于行为刺激的动物的区域特异性神经化学变化 活性组分。 或者，如果（2）中获得的结果表明 未分级溶剂提取物不会产生相当于 我们会进行研究， 内源性（脂肪酸、硒）和外源性（甲基汞、砷、铅） 必须将不可浸提组分加入溶剂浸提物中， 行为等价 这些实验将使我们能够确定 污染物的种类是造成神经心理变化的原因 在围产期暴露的人身上看到的。 这些发现将有助于降低 评估过程，也将成为以后研究的基础， 确定行为改变的机制。

DESCRIPTION: (Adapted from the Investigator's Abstract) Recent epidemiological studies suggest a relationship between consumption of contaminated Great Lakes fish by pregnant women and developmental and cognitive deficits in their infants and children. Although polychlorinated biphenyl (PCB) exposure is correlated with these dysfunctions, recent data we have collected from rats perinatally exposed to diets containing lyophilized Great Lakes salmon suggest that the observed neuropsychological effects may be due to other neuroactive contaminants in the fish or to synergistic interactions between PCBs and other contaminants. In order to determine which toxicants present in Great Lakes fish induce neurobehavioral alterations following perinatal exposure we will: (1) measure concentrations of the organic and inorganic contaminants in Lake Ontario (LO) salmon and in Pacific Ocean (PO) salmon, which will serve as a control in all experiments; (2) determine neurobehavioral effects in rats perinatally exposed to lyophilized LO salmon (contains all contaminants) or a sulfuric acid extract of LO salmon (contains all organic contaminants). If the neurobehavioral effects of the lyophilized LO salmon and the solvent extract are equal we will assume that the non-extractable components (including MeHg) are not involved in mediating the effects and we will go on to; (3) determine the neurobehavioral effects of two florisil column fractions of the LO solvent extract -- one containing the majority of the PCBs, DDE, HCB, mirex and chlordanes, and the other containing the coplanar PCBs, dioxins, dibenzofurans and polar pesticides and; (4) determine regio-specific neurochemical changes in animals exposed to the behaviorally active fraction(s). Alternatively, if the results obtained in (2) indicate that the unfractionated solvent extract does not yield effects equivalent to those of the lyophilized LO salmon, we will conduct studies to determine which endogenous (fatty acids, selenium) and exogenous (MeHg, arsenic, lead) non-extractable components must be added to the solvent extract to result in behavioral equivalence. These experiments will allow us to determine which class(es) of contaminants are responsible for the neuropsychological changes seen in perinatally-exposed humans. The findings will aid in the risk assessment process and will also form the basis for later studies to determine the mechanisms responsible for the behavioral alterations.