CORE--PRODUCTION OF C PARVUM OOCYSTS

获得完全孢子化卵囊的困难 体外培养意味着卵囊和子孢子必须仍然是 从受感染的动物身上获得。 最大的数字可以很容易地 从受感染的生闷气的老小牛身上获得。 卵囊技术 保存、分离和纯化是公认的。 最近， 已经描述和改进了用于分离 感染动物的裂殖子 体外培养的改进 技术可能会进一步影响这种无性繁殖的隔离， 生命周期阶段 核心9001计划项目是专门为提供 寄生虫试剂，包括卵囊、子孢子和裂殖子， 用于项目#1-4和核心9002的微小隐孢子虫 根据每个项目的需求制定项目计划。 没有这个核心 就不会有项目计划。

Difficulties attendant with obtaining completely sporulated oocysts from in vitro cultures means that oocysts and sporozoites must still be obtained from infected animals. The greatest numbers can most easily be obtained from infected sulking aged calves. Techniques for oocyst preservation, isolation and purification are well established. Recently, techniques have been described and improved upon for the isolation of merozoites from infected animals. Improvements of in vitro cultivation techniques will likely further impact on the isolation of this asexual life cycle stage. Core 9001 of the Program Project is designed specifically to provide parasite reagents including oocysts, sporozoites, and merozoites of Cryptosporidium parvum for use in Projects #1-4 and core 9002 of the Program Project as prescribed by the needs of each. Without this Core, there would be no Program Project.