ZINC KINETICS IN METALLOTHIONEIN KNOCKOUT MICE

本申请提出研究金属硫蛋白（MTI） 和金属硫蛋白-II（MTII）在MTI/MTII无效或敲除小鼠锌 动力学 目的是利用PI长期以来的矿物建模 使用SAAM的专业知识，以及先前开发的Zn动力学模型 在野生型大鼠中研究MTs在锌调节中的作用 吸收和分配。 假设MT将在 在体内平衡，虽然这种影响的网站是不清楚的， 在提案中确认。 具体地，野生型和无效小鼠将 饲喂含锌35或100 mg/kg的饲料， 将口服65 Zn，一半将静脉注射65 Zn。 将在8天内跟踪锌动力学。 具体 研究的目的是：1）确定锌动力学的差异， 删除两个主要MT; 2）使用这些差异来识别 MT在锌稳态中的作用。

This application proposes to study the impact of metallothionein-(MTI) and metallothionein-II (MTII) in MTI/MTII null or knockout mice on Zn kinetics. The aim is to use the PI's long-standing mineral modeling expertise using SAAM, and a previously developed model for Zn kinetics in wild-type rats to study the role of MTs in the regulation of Zn absorption and distribution. The hypothesis is that MT will have a role in homeostasis, although the site of this impact is not clearly identified in the proposal. Specifically, wild-type and null mice will be fed diets containing 35 or 100 mg Zn/kg diet, and half of the animals will receive 65Zn orally and half will receive 65Zn by i.v. injection. Zn kinetics will be followed over an eight day period. The specific aims of the study are: 1) to identify differences in Zn kinetics due to deletion of the two major MTs; 2) to use these differences to identify a role for MT in Zn homeostasis.