HISTOLOGICAL EXAM OF BIOLOGICAL INTERACTIONS OF GLOXY W/ MICE TISSUES

Gloxy是一种具有EPR信号的煤， 对氧分压(PO2)的变化敏感。它有 有可能成为主要的顺磁材料之一 将用于拓宽EPR血氧测定仪的生活领域 研究对象。对组织对存在的反应的评估 对这种材料的有效利用是必不可少的。这样做的目的是 研究是为了确定生物相互作用的组织学证据 的组织，以存在植入的Gloxy和长期 在组织中对氧的稳定性敏感。格洛西是 植入脑、肾、肝和股四头肌 老鼠。植入后，含有Gloxy的组织 在特定的终点收获，组织学检查 通过光学和电子显微镜进行。定期EPR 光谱是从活体动物身上获得的。组织学评估 表明Gloxy相当稳定，不会引起显著的 在这段时间内，这项研究检查的组织中的反应 观察的能力。24小时。其中一些样本确实有证据表明 出血似乎与身体创伤有关 植入物和一些炎性细胞也存在。七 植入几天后，没有出血的迹象， 至多可见少量炎性细胞。 在整个观察期内，没有证据表明 粘液部位的炎症或异物组织反应。 这两种形式的Gloxy至少保持了对氧气的敏感性 在整个实验期间(6个月)，在所有不同的器官( 脑、肾、肝、肌肉)。

Gloxy is a type of coal which has an EPR signal which is very sensitive to changes of the partial pressure of oxygen (pO2). It has the potential to be one of the principal paramagnetic materials that will be used for the widening field of EPR oximetry in living subjects. An assessment of the responses of tissues to the presence of this material is essential for its effective use. The aim of this study is to ascertain histological evidence of biological interaction of the tissues to the presence of the implanted gloxy and long-term stability in tissues of the sensitivity to oxygen. Gloxy was implanted into the brain, kidney, liver, and quadriceps muscle of mice. After implantation, the tissues containing the gloxy were harvested at specific endpoints and histological examination was carried out by light and electron microscopy. Periodically EPR spectra were obtained from the live animals. Histological assessments demonstrated that gloxy is quite stable and did not induce significant reactions in the tissues examined in this study during the time period of observation. At 24 hr. some of the samples did have evidence of hemorrhage which appeared to be associated with the physical trauma of implantation and some inflammatory cells also were present. Seven days after the implantation, there was no indication of hemorrhage and at most, a small amount of inflammatory cells was observed. Throughout the observation period there was no evidence of significant inflammation or foreign tissue reaction at the site of the gloxy. Both forms of gloxy remained their sensitivity to oxygen at least throughout the experiment ( 6 months) in all of the various organs ( brain, kidney, liver, muscle).