METABOLIC FATE PLASMA KINETICS OF DIETARY SOY ISOFLAVONE

描述：（申请人的描述）意识到饮食在 在西方人群的许多常见疾病中起着重要作用， 对食品中多种生物活性非营养物质的认识。 之间 这些植物雌激素在植物中相对丰富， 在远东地区， 癌症和癌症依赖性疾病的发病率明显低于 西方人口。 大豆是一个丰富的糖苷来源， 异黄酮、大豆苷元和染料木黄酮，它们是雌激素模拟物， 无数有效的生物学特性可以解释它们的作用 明显的抗癌作用。 现在很明显， 植物雌激素可能对人类疾病有显著的益处 预防 虽然已知大豆异黄酮是通过 肠道细菌，吸收，结合在肝脏，循环， 血浆中，并在尿液中排泄，这些信息大部分是定性的， 自然 这个应用程序旨在提高我们对这些因素的理解 确定血浆水平，生物利用度和动力学的 女性和男性的性别差异，这些是如何受到类型的影响， 基质的量，化学成分，是否有性别或 年龄相关的代谢和生物利用度差异，如果肠道 运输影响新陈代谢。 血浆和尿液中的肌酐浓度 将通过质谱法测量，这是唯一具有所需 灵敏度、精密度和特异性， 在大的动态范围内的生物流体。 我们建议设立 大豆异黄酮的药代动力学的剂量-反应关系， 大豆食品的营养正常摄入范围，并比较 血浆和尿液中的浓度达到了三个不同的 含有一定水平的大豆酮的大豆食品， 对人类和动物的生物学影响。 肠道转运将是 测量以检查停留时间对代谢的影响， 吸收ECONONE。 尿液与血浆的相关性 浓度将允许评价最适当的方法， 评估大豆食品的合规性。 这些研究将改善我们的 了解大豆食品在多大程度上提供生理 相关级别的信息，将有助于设计 未来的饮食研究。

DESCRIPTION: (Applicant's Description) The awareness that diet plays an important role in many common diseases of Western populations, has led to the recognition of many classes of bioactive non-nutrients in foods. Among these, the phytoestrogens are relatively abundant in plants, and occur in high concentrations in foods consumed in the Far-East, where the prevalence of cancers and hormone-dependent diseases is significantly lower than in Western populations. The soybean is a rich source of glycosides of the isoflaones, daidzein and genistein, which are estrogen mimics, and possess a myriad of potent biological properties that can account for their demonstrable anticancer actions. It is now apparent that a diet rich in phytoestrogens may confer significant benefit to humans in disease prevention. While it is known that soy isoflavones are metabolized by intestinal bacteria, absorbed, conjugated in the liver, circulated in plasma, and excreted in urine, much of this information is of a qualitative nature. This application seeks to improve our understanding of the factors that determine the plasma levels, bioavailability, and kinetics of isoflavones in women and men, how these are influenced by the type and amount of the matrix, and chemical composition, whether there are gender or age-related differences in metabolism and bioavailabilly and if intestinal transit influences metabolism. Plasma and urinary isoflavone concentrations will be measured by mass spectrometry, the only method with the required sensitivity, precision and specificity for the detection of isoflavones in biological fluids over a large dynamic range. We propose to establish dose-response relationships for the pharmacokinetics of soy isoflavones in a nutritionally normal range of intake, for a soy food, and to compare the plasma and urinary isoflavone concentrations attained with three different soy foods containing a level of isoflavones previously found to have biological effects in humans and animals. Intestinal transit will be measured to examine the effect of residence time on metabolism and absorption of isoflavones. Correlation between urinary and plasma concentrations will permit an evaluation of the most appropriate means of assessing compliance to soy foods. These studies will improve our understanding of the extent to which soy-foods deliver physiologically relevant levels of isoflavones, information that will facilitate the design of future dietary studies.