INFERIOR COLLICULUS CALCIUM CURRENT AND SEIZURE

癫痫是神经元兴奋性的紊乱。 在细胞 水平，癫痫发作活动由去极化电压序列组成 转移（DS），其需要钙（Ca）流入。 长期 该项目的目标是确定细胞和分子 癫痫活动背后的蛋白质变化。 的目的 建议是表征钙电流的哪些方面已经被 乙醇戒断（ETX）和听源性癫痫发作（AGS）后的变化 在ETX之后，全身性癫痫的模型。 的具体目标 本研究的建议是：1）从生物学和药理学的角度 识别急性分离的下丘脑-垂体-肾上腺皮质--肾上腺皮质-肾上腺皮质- 丘（IC）神经元使用膜片钳技术，2）表征 电压对IC N型和L型钙通道活性的影响 3）检测ETX事件和AGS对Ca电流的影响 4)为了鉴定哪种Ca通道亚基在IC中表达， 分子技术4）以确定ETX和/或AGS是否改变Ca亚基 mRNA水平。确定Ca内流在ETX机制中的作用 IC中的癫痫发作可能与临床全身性癫痫发作相关， 强直阵挛性运动行为 了解细胞和分子 癫痫的基础将改善治疗策略以及产量 对大脑功能和结构的新见解。

Epilepsies are disorders of neuronal excitability. At the cellular level, seizure activity consists of trains of depolarizing voltage shifts (DS) which requires calcium (Ca) influx. The long-term objectives of this project are to identify the cellular and molecular changes in proteins underlying seizure activity. The aim of this proposal is to characterize which aspects of Ca currents have been changed after ethanol withdrawal (ETX), and audiogenic seizure (AGS) following ETX, a model of generalized epilepsy. The specific aims of this research proposal are 1)to pharmacologically and biophysically identify the types of Ca currents in acutely dissociated inferior colliculus (IC) neurons using patch-clamp techniques, 2) to characterize the effects of voltage on N- and L-type Ca channel activity in IC neurons 3) to examine the effects of ETX episode, and AGS on Ca currents 4) to identify which Ca channel subunit are expressed in the IC using molecular techniques 4) to determine if ETX and/or AGS alter Ca subunit mRNA levels. Determining a role for Ca influx in the mechanism of ETX seizure in the IC may be relevant to clinical generalized seizure with tonic-clonic motor behaviors. Understanding the cellular and molecular basis of epilepsy will improve therapeutic strategies as well as yield new insights into brain function and structure.