DESIGN AND IMPLEMENTATION OF NEW BIOCOMPATIBLE MATERIALS

本提案的目的是研究以下因素的相互作用： 具有合成界面的蛋白质， 它们能抵抗蛋白质的吸附， 这些表面防止蛋白质吸附。 此外该 嵌入惰性表面的底物的水解， 将研究表面以上溶液中的酶， 蛋白质与抵抗其吸附的表面相互作用。 蛋白质在生物医学植入物表面的吸附可以 促进微生物在表面以及其它 导致设备故障的有害影响。 换句 在某些应用中，蛋白质吸附到合成材料是有用的。 的 操纵蛋白质吸附的能力是重要的一步， 在控制对合成表面的生物反应方面， 生物相容性材料，并确定新的应用， 生物学、生物化学和医学中的生物材料。 所采用的研究方法包括使用自组装 单层（SAMs）作为定义明确的模型表面、表面等离子体 共振（SPR）光谱作为一种方便的分析技术， 对蛋白质吸附呈惰性的合成表面。 这 方法的组合是唯一适合研究生物学的方法。 合成表面化学

The objective of this proposal is to investigate the interaction of proteins with synthetic interfaces in order to identify new materials that resist the adsorption of proteins and understand the mechanisms by which these surfaces prevent protein adsorption. In addition, the hydrolysis of substrates that are embedded in an inert surface by enzymes in solution above the surface will be studied to understand how proteins interact with surfaces that resist their adsorption. The adsorption of proteins on the surface of biomedical implants can facilitate microbial colonization of the surface as well as other deleterious effects that result in the failure of the device. In other applications, protein adsorption to synthetic materials is useful. The ability to manipulate protein adsorption represents an important step in controlling biological responses to synthetic surfaces, designing new biocompatible materials, and identifying new applications for biomaterials in biology, biochemistry, and medicine. The research methods to be applied consist of using self-assembled monolayers (SAMs) as well-defined model surfaces, surface plasmon resonance (SPR) spectroscopy as a convenient analytical technique, and synthetic surfaces that are inert toward protein adsorption. This combination of methods is uniquely suited to study the biological chemistry of synthetic surfaces.