FLOW CYTOMETRIC ANALYSIS OF GENOTOXICITY

描述：（改编自申请人摘要）：本研究的目的 建议开发一种低成本、快速、可靠和灵敏的流动方法， 确定诱变。该系统基于CHO AL细胞系，其具有 人类11号染色体11号染色体上的突变可以通过缺失 11号染色体上编码的表面抗原的表达。前提是 11号染色体上的突变可以准确、灵敏和快速地检测出来， 通过基于表面抗原的损失的流式细胞术。的具体目标 该建议是开发用于染色CHO AL细胞的最佳方法， 抗体来测量S1抗原的存在或不存在： 流式细胞术诱变试验的灵敏度;通过以下方法验证试验 将用流式细胞术测量的突变体级分与用流式细胞术发现的突变体级分进行比较。 标准分析;并确定设计参数，用于小型，可靠 廉价、高分辨率、低噪音、快速流式细胞仪，使常规 突变频率的测量。制定这一方法将 允许快速和廉价地筛选药物的遗传毒性。 拟议商业应用：不可用

DESCRIPTION: (Adapted from applicant's abstract): The objective of this proposal is to develop a low cost, rapid, reliable and sensitive flow method to determine mutagenesis. The system is based on a CHO AL cell line, which has human chromosome 11. Mutations in chromosome 11 are detected by loss of expression of surface antigens encoded on chromosome 11. The hypothesis is that mutations in chromosome 11 can be accurately, sensitively and rapidly detected by flow cytometry based on the loss of surface antigens. The specific aims of this proposal are to develop optimal methods for staining CHO AL cells with antibodies to measure the presence or absence of S1 antigen: determine the sensitivity of the flow cytometry mutagenesis assay; validate the assay by comparing mutant fractions measured with flow cytometry to that found by the standard assay; and identify design parameters for a small, reliable inexpensive, high resolution, low noise, fast flow cytometer to make routine measurements of mutant frequency. The development of this methodology would allow for rapid and inexpensive screening pharmaceuticals for genotoxicity. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE