ISOLATION AND PROFILING OF CIRCULATING PC CELLS IN BLOOD

该项目的主要长期目标是1)标准化个体前列腺癌患者癌细胞的培养，2)开发癌症预测特征的分子谱，如染色体非整倍体，细胞增殖和静止的循环标记，雄激素受体基因拷贝。分子谱的目的是提供一个窗口到循环癌细胞的转移潜力，作为预后工具。患者特异性培养细胞，除了为研究前列腺癌的研究人员提供丰富的研究材料外，还将服务于（体外体外）的巨大临床市场。第一阶段的具体目标是：1。将培养的前列腺癌细胞植入富含白细胞的部分（模拟白细胞分离——浓缩循环癌细胞），并富集活的癌细胞。2. 比较进一步富集的实验方法（癌细胞与白细胞的比例为1:10）。3. 培养癌细胞。4. 开发用于这些样品的分子谱分析。5. 最终目的是从15例白血病患者的样本中分离、进行分子表征和培养癌细胞。分子分析领域的技术创新是一个分离和表征循环癌细胞的系统，并为进一步的工作提供循环肿瘤培养物。建议的商业应用：直接从患者血液中分离癌细胞的分子分析作为预后的临床测试和/或直接监测治疗对循环癌细胞的影响，可能具有很大的商业影响。第二个主要的商业潜力将是从个体患者身上分离出的癌细胞培养物的应用。

The major long term goals of this project are 1) to standardize culture of cancer cells from individual prostate cancer patients, 2) to develop molecular profiling for cancer predictive characteristic such as chromosomal aneuploidy, circulating markers for cell proliferation and quiescence, androgen receptor gene copy. The molecular profiling is intended to provide a window into the metastatic potential of circulating cancer cell, as a prognostic tool. The patient-specific cultured cells, besides providing a wealth of research material for researchers studying prostate cancer, would serve the vast clinical market for (in-vitro out of body). Specific aims of Phase 1 are: 1. Seed cultured prostate cancer cells into white blood cell rich fraction (simulating leukapheresis-which concentrates circulating cancer cells) and enrich for live cancer cells. 2. Compare experimental approaches to further enrichment (to a 1:10 cancer cell to WBC ratio). 3. Culture the cancer cells. 4. Develop molecular profile assays for use on these samples. 5. Final goal is isolate, perform molecular characterization and culture cancer cells from 15 patients' leukaphereis samples. The technological innovation to the field of molecular analysis is a system to isolate, and characterize circulating cancer cells and provide circulating cancer cultures for further work. PROPOSED COMMERCIAL APPLICATION: Molecular profiling of cancer cells isolated directly from a patient's blood could have great commercial impact as a clinical test for prognosis and/or to directly monitor the effects of therapy on the circulating cancer cells. The second major commercial potential would be applications for cancer cell cultures isolated from individual patients.