NEURAL DEVELOPMENT AND GENETICS OF ZEBRAFISH

建议为高级研究生开设为期两周的强化课程， 医学生、博士后研究员、医学研究员和 独立调查人员将专注于发展和 斑马鱼的遗传学，特别强调神经系统。 的 课程的第一周将涵盖早期发展和遗传学 斑马鱼和相关的方法，而第二周 将专注于神经系统。 上午将用于讲座 和讨论，下午到3-4小时的实验室练习。 每个 一天将在一名高级教员的监督下， 由一名或两名以上的初级教员协助。 该课程将 仅限12-16名学生。 第一周的讲座将涵盖以下发展阶段： 斑马鱼，化学和插入突变，早期发育和 器官发生和斑马鱼饲养。 第二周，学生们 将被引入斑马鱼的神经系统， 以及神经系统突变体的分析 具体领域将 第二周包括前脑和后脑，脊髓， 眼睛和视网膜，顶盖和斑马鱼行为的各个方面。 实验室 第一周的练习将向学生介绍如何发展野生动物- 类型和突变胚胎，核酸注射技术，细胞 移植、原位杂交和标记单个 细胞 在第二周，学生们将解剖斑马鱼神经 系统，研究脑组织学，对野生型进行行为测试， 突变动物，学习轴突寻路技术。

An intensive two-week course is proposed for advanced graduate students, medical students, postdoctoral fellows, medical research fellows and independent investigators that will focus on the development and genetics of zebrafish with special emphasis on the nervous system. The first week of the course will cover the early development and genetics of zebrafish and the relevant methodologies, whereas the second week will focus on the nervous system. Mornings will be devoted to lectures and discussion and afternoons to 3-4 hour laboratory exercises. Each day will be under the supervision of a senior faculty member who will be assisted by one or two more junior faculty members. The course will be limited to 12-16 students. Lectures in the first week will cover the developmental stages of zebrafish, chemical and insertional mutagenesis, early development and organogenesis and zebrafish husbandry. In the second week, the students will be introduced to the nervous system of zebrafish, its development and the analysis of nervous system mutants. Specific areas to be covered in the second week include the fore- and hindbrain, spinal cord, eye and retina, tectum and aspects of zebrafish behavior. Laboratory exercises in the first week will introduce students to developing wild- type and mutant embryos, techniques of nucleic acid injection, cell transplantation, in situ hybridization, and the labeling of single cells. In the second week, students will dissect the zebrafish nervous system, study brain histology, perform behavioral tests on wild-type and mutant animals, and learn axonal pathfinding techniques.