CRPV MODULATION OF KERATINOCYTE CD40 EXPRESSION

描述：本拨款申请调查抗病毒药物的性质。 宿主对乳头状瘤病毒的免疫应答，特别是棉尾鼠 兔乳头瘤病毒（CRPV）。具体来说，PI描述了实验 研究CRPV感染是否导致表达调节， CD40在兔角质形成细胞中的表达。这种T淋巴细胞共刺激因子 最近在人角质形成细胞中检测到这种分子。因此 PI假设乳头瘤病毒对其表达的调节 感染可以改变宿主的免疫反应。该补助金的三个目标 是1）克隆CD40的兔同源物，2）确定是否 兔CD40在兔角质形成细胞中表达，以及3）确定 是否在感染CRPV的组织中表达， 这种表达在那些代表持续性的病变中不同， 感染，或进行性病变与消退性病变。

DESCRIPTION: This grant application investigates the nature of anti-viral host immune responses against papillomaviruses, specifically cottontail rabbit papillomavirus ( CRPV). Specifically, the PI describes experiments to investigate whether CRPV infection leads to a modulation of expression of CD40 in rabbit keratinocytes. This T-lymphocyte co-stimulatory molecules has recently been detected in human keratinocytes. Therefore the PI hypothesizes that modulation of its expression by papillomavirus infection could alter host immune responses. The three aims of this grant are 1) to clone the rabbit homologue of CD40, 2) to establish whether rabbit CD40 is expressed in rabbit keratinocytes, and 3) to determine whether its expression is present in tissue infected with CRPV and whether this expression differs in those lesions that represent persistent infections, or progressing versus regressing lesions.