GENETIC ANALYSIS OF VERTEBRATE RETINA DEVELOPMENT

描述(摘自申请者摘要)：长期目标 建议的研究是为了揭示细胞的遗传和分子机制。 脊椎动物视网膜的分化。影响基因的隐性突变 斑马鱼(Danio Rerio)眼睛的发育是从一个 化学诱变筛选，申请人建议进一步 描述这些突变品系的特征。第一个目标是检查突变体 通过组织学和免疫学分析，选择几条线 以作进一步分析。第二个目标是定义突变的 通过遗传镶嵌分析获得的基因(将突变的细胞移植到 野生型胚胎)和互补分析(寻找其他 用基因方法检测突变基因的等位基因)。

DESCRIPTION (Adapted from applicant's abstract): The long-term objective of the proposed research is to uncover genetic and molecular mechanisms of cell differentiation in the vertebrate retina. Recessive mutations affecting the development of the eye in zebrafish (Danio rerio) have been recovered from a chemical mutagenesis screen, and the applicant proposes to further characterize these mutant lines. The first aim is to examine the mutant eyes by histological and immunological analysis, and to select a few lines for further analysis. The second aim is to define the nature of the mutated genes by genetic mosaic analysis (transplanting cells from mutant into wildtype embryos) and by complementation analysis (searching for additional alleles of the mutated genes by genetic methods).