HYBRID RETROVIRUS AND ITS REPLICATION

该项目的目的是构建和表征一种新型的 混合逆转录病毒将使用一种新的复制策略。这 复制策略将使病毒的使用更安全，无论是作为 治疗性或预防性的工具。这是这次活动的具体目的。 改造这种病毒以允许在人类细胞中复制的项目。 该项目的总体目标是使这种混合病毒适应于 用于基因治疗和疫苗。因为显而易见的重要性 对公共卫生和药物的安全问题，这些新的逆转录病毒 实体将在生物技术和医学领域有广泛的应用 更安全，也许更有效的载体，用于将基因转移到 需要基因治疗和作为更安全的疫苗的动物和人类 病毒性疾病和癌症。杂交病毒将被构建并 使用标准的分子生物学技术进行测试，他们将 完全由可获得的DNA质粒序列组成。这些小说 逆转录病毒将开启逆转录病毒研究的新领域 复制将导致更好地理解这一过程 并有可能导致新的、更有效的方法 病毒性疾病的治疗。 建议的商业应用： 这项研究的潜在商业应用是：在健康方面 护理和药物，更具体地说，在基因治疗中，抗病毒 疫苗和癌症疫苗。

The aim of this project is to construct and characterize a new type of hybrid retrovirus that will use a novel replication strategy. This replication strategy will make the virus safer for use, either as a therapeutic or a preventive tool. It is the specific aim of this project to engineer this virus to allow for replication in human cells. The general objective of the project is to adapt this hybrid virus for use in gene therapy and for vaccines. Because of the obvious importance of safety issues to public health and medicine, these new retroviral entities will have broad application in biotechnology and medicine as safer, and perhaps more efficient, vectors for transfer of genes into animals and humans in need of gene therapy and as safer vaccines against viral disease and cancer. The hybrid viruses will be constructed and tested using standard molecular biology techniques, and they will consist entirely of available DNA plasmid sequences. These novel retroviruses will open a new field into research of retroviral replication that will result in a better understanding of this process and could potentially lead to a new and more efficient approaches in treatment of viral diseases. PROPOSED COMMERCIAL APPLICATION: The potential commercial applications of this research are: in health care and medicine, more specifically in gene therapy, anti-viral vaccines, and cancer vaccines.