DEVELOPMENT OF A NOVEL HPV IDENTIFICATION SYSTEM

描述（改编自研究者摘要）：浸润性癌 宫颈癌是世界上最常见的癌症之一。 人类 乳头瘤病毒（HPV），通过宫颈上皮内 肿瘤，是发展侵袭性肿瘤的主要原因。 宫颈癌 然而，在70种或更多的HPV亚型中， 到癌症 虽然早期发现加上根除 子宫颈癌前体已被证明会导致减少 浸润性宫颈癌的发病率，最新技术水平 采用的诊断技术成本太高或太耗时 来有效地筛选大量人群。 准确的HPV毒株 目前只能通过繁琐DNA鉴定 杂交分析利用Southern印迹技术或 原位杂交技术 在下面的提议中，一种酶- 将开发辅助的序列特异性鉴定系统。 简单、快速、灵敏、准确、低成本的DNA诊断 本文所述系统将在HPV感染的非常早期阶段检测HPV， 感染，并将区分低致癌风险菌株和 高致癌风险菌株。 这将使大规模筛选， 建立一个敏感、快速和 相对便宜。 本提案所描述的诊断工具 也适用于其他传染病和遗传病的检测 只有轻微的变化。 拟议商业应用：不可用

DESCRIPTION (Adapted from investigator's abstract): Invasive carcinoma of the cervix is one of the most common cancers in the world. Human papillomavirus (HPV), progressing through cervical intraepithelial neoplasia, is the primary causal agent for the development of invasive cervical cancer. However, of the 70 or more HPV subtypes, few progress to cancer. Although early detection coupled with eradication of cervical cancer precursors has been shown to lead to a decreased incidence of invasive cervical cancer, the present state of the art employs diagnostic techniques that are too costly or too time consuming to screen large populations efficiently. Accurate HPV strain identification is presently only possible through cumbersome DNA hybridization analysis utilizing either Southern blot technology or in situ hybridization technology. In the following proposal, an enzyme- assisted, sequence-specific identification system will be developed. The simple, rapid, sensitive, accurate, and low-cost DNA diagnostic system described herein will detect HPV at very early stages of infection and will distinguish between low oncogenic risk strains and high oncogenic risk strains. This will enable large scale screening by establishing a diagnostic protocol that is sensitive, rapid, and relatively inexpensive. The diagnostic tool described by this proposal is also applicable to detection of other infectious diseases and genetic diseases with only minor modifications. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE