PERMEABILITY CHARACTERISTICS OF DENTIN

描述(改编自研究人员摘要)：牙本质粘结 通常涉及使用酸性调理剂来去除污渍层 使牙本质表面脱矿，露出下面的胶原纤维 肾小管间牙本质基质。这个脱矿基质具有如此低的 如果允许风干，它可能会崩溃的弹性模数，这是一种常见的 临床程序。当它坍塌时，胶原纤维之间的间隙 被认为会变得越来越小，而且渗透性可能会降低， 然而，正是通过这些通道，树脂在树脂过程中必须扩散 凝聚力。如果脱钙基质的弹性模数可以是 增加到临界值以上时，网络可能不会在空气中崩溃 干燥，从而保持对树脂单体的渗透性。各种各样的 将对脱矿牙本质硬化的能力进行评估 矩阵。到目前为止，还没有人开发出量化方法来衡量 树脂对脱矿牙本质的渗透作用。的渗透性 将测量湿基质和干基质中脱矿牙本质到树脂的比例。 干缩的纤维网络被认为对树脂的渗透性较差 单体比湿的、扩大的网络要好。拟议的研究将检验这一点 假设。不同的酸调节剂对渗透性的影响， 胶原蛋白的拉伸性能、尺寸和溶解性将在 脱矿牙本质和肌腱都是不溶性胶原的例子 矩阵。几种不同的盐类添加剂将添加到酸性调理剂中 进行评估，看看哪一种最能保护胶原蛋白免于变性。 脱矿牙本质基质随时间变化的稳定性知之甚少 在水里。脱矿牙本质基质的长期稳定性 在没有树脂渗透的情况下，在水溶液和非水溶液中， 评估对基质可能的水解性降解的关注，并 随着时间的推移，拉伸性能的损失。这些宏观结果将是 用扫描电子显微镜和原子力在微观层面上重新审视 用来测量弹性模数的改装显微镜。这些研究的结果 研究将为改进的酸性制剂提供新的信息 护发素，脱矿的牙本质应该如何用底漆处理，如何 油井底漆树脂穿透塌陷或膨胀的脱矿牙本质，以及 它们密封和保护胶原纤维的效果如何。结果也将是 为模拟管间渗透率特性提供数据 以及管状牙本质。

DESCRIPTION (Adapted from the investigator's Abstract): Dentin bonding generally involves the use of acid conditioners to remove the smear layer and demineralize the dentin surface to expose the underlying collagen fibers of the intertubular dentin matrix. This demineralized matrix has such a low modulus of elasticity that it can collapse if allowed to air-dry, a common clinical procedure. As it collapses, the spaces between the collagen fibers are thought to become progressively smaller and presumably less permeable, yet it is through these channels that resins must diffuse during resin bonding. If the modulus of elasticity of the demineralized matrix can be increased above a critical value, the network may not collapse when air dried, thereby remaining permeable to resin monomers. A variety of procedures will be evaluated for the ability to stiffen demineralized dentin matrix. Thus far, no one has developed quantitative methods to measure the permeation of resin into demineralized dentin. The permeability of demineralized dentin to resins in wet versus dry matrices will be measured. Dry collapsed fiber networks are thought to be less permeable to resin monomers than wet, expanded networks. The proposed studies will test that hypothesis. The effects of different acid conditioners on the permeability, tensile properties, dimensions and solubility of collagen will be tested on both demineralized dentin and tendon as examples of insoluble collagen matrices. Several different salt additives to the acidic conditioners will be evaluated to see which ones best protect collagen from denaturation. Little is known about the stability of demineralized dentin matrix over time in water. The long-term stability of demineralized dentin matrix, with or without resin infiltration, in aqueous and nonaqueous solutions, will evaluate concerns about possible hydrolytic degradation of the matrix and loss of tensile properties over time. These macroscopic results will be re-examined at the microscopic level using both SEM and an atomic force microscope modified to measure modulus of elasticity. The results of these studies will provide new information on improved formulations of acidic conditioners, how demineralized dentin should be treated with primers, how well primer resins penetrate collapsed or expanded demineralized dentin, and how well they seal and protect the collagen fibers. The results will also provide data for modeling the permeability characteristics of intertubular as well as tubular dentin.