ADVANCED MEMS FOR HIGH THROUGHPUT DNA SEQUENCING

人类基因组计划的目标是完成基因组测序 人类和几个模型系统。 为了实现这一目标， 必须在开发和应用方面取得重大进展， 几个关键技术。 低成本、高速DNA测序和 基因分型是这些技术之一。在两年半的时间里， 我们目前的微型DNA电泳芯片计划，我们 已经成功地快速开发和常规操作测序 装置.我们的微芯片设备已经证明了这项技术的可行性， 已经为这类设备的性能设定了标准。基于 根据我们的研究结果和我们的多学科专业知识，我们提出了三个 一项为期一年的计划，建立和测试384道显微DNA分析 在现实的基因组中心条件下的系统。 系统应 在序列生产中提供至少一个数量级的增加 相应地降低了总测序成本。

The goal of the Human Genome Project is to complete the genome sequence of humans and several model systems. To achieve this goal, order-of- magnitude advances must be made in the development and application of several key technologies. Low cost, high speed DNA sequencing and genotyping is one of these technologies. In the two and one half years of our current program on microfabricated DNA electrophoresis chips, we have succeeded in rapidly developing and routinely operating sequencing devices. Our microchip devices have proved the technology works and we have set the standards for performance of this class of devices. Based on our results and our multidisciplinary expertise, we propose a three year program to build and test 384-lane microfabricated DNA analysis systems under realistic genome center conditions. The systems should provide at least an order of magnitude increase in sequence production with corresponding decreases in overall sequencing costs.