PEPTIDE HYDROLYSIS BY DINUCLEAR METAL COMPLEXES

本提案的目的是了解双核金属水解酶的酰胺水解机制以及配位环境与金属中心活性之间的关系。 这将通过使用合成的模型复合物模拟双核金属水解酶的活性位点来完成。 一个可调的，双核酞嗪为基础的配体系统已被设计用于这项研究。 这些配体的双核配合物将用于探测所提出的机制的各个方面，并且将调整配体环境以优化水解活性。 水解可行的双核金属配合物的这项研究将有助于阐明水解金属酶的机制，澄清在这些系统中的金属离子的作用，并最终帮助人工水解酶的发展。

The objectives of this proposal are to gain an understanding of the mechanism of amide hydrolysis by dinuclear metallohydrolases and the relationship between the coordination environment and the activity of the metal center. This will be done by modeling the active sites of dinuclear metallohydrolase enzymes using synthetic model complexes. A tunable, dinucleating phthalazine-based ligand system has been designed for use in this study. Dinuclear complexes of these ligands will be used to probe aspects of the proposed mechanism, and the ligand environment will be tuned to optimize hydrolytic activity. This study of hydrolytically viable dinuclear metal complexes will aid in the elucidation of hydrolytic metalloenzyme mechanisms, clarify the roles of the metal ions in these systems, and ultimately aid in the development of artificial hydrolases.