POSTNATAL NEURONAL PRECURSORS AND BRAIN REPAIR

描述：幼年和成年哺乳动物的脑室下带(SVZ) 大脑，包括灵长类动物，包含一个迅速增殖的种群 细胞。新的神经元和胶质细胞可以从这些增殖的SVZ中衍生出来 成年小鼠体内的前体。成人SVZ细胞也可以在体外培养 这些细胞具有形成神经元和神经胶质细胞的潜能。 体外培养。在啮齿动物中，SVZ细胞迁移到嗅球几毫米，在那里 它们分化为神经元。这些SVZ细胞迁移与 相互作用，形成神经元前体链(链迁移)， 忠实地到达嗅球，而不是突起的胶质或轴突 指导。深圳开发区存在广泛的连锁迁徙网络 成年小鼠表明史无前例的神经元前体交通 存在于成年哺乳动物的大脑中。目标是确定潜在的 以产生新的神经元用于大脑修复。为此， 将解决以下问题。1)需要嗅球吗？ SVZ前驱体的迁移和增殖？2)SVZ前驱体 移植到脊髓不同区域后产生新的神经元 成人大脑？3)成人大脑中是否存在神经前体细胞链？ 其他成年脊椎动物，包括灵长类和人类？5种中的哪一种？ 研究人员最近在SVZ中描述的细胞类型 成年小鼠在体外与表皮生长因子或碱性成纤维细胞生长因子？5)SVZ前体细胞 直接从脑中分离或与生长因子一起体外培养 移植后整合和生成不同类型的神经元 胚胎大脑？

DESCRIPTION: The subventricular zone (SVZ) in juvenile and adult mammalian brains, including primates, contain a population of rapidly proliferative cells. New neurons and glia can be derived from these proliferating SVZ precursors in adult mice. Adult SVZ cells can also be grown in vitro with EGF or bFGF and these cells retain the potential to form neurons and glia in vitro. In rodents, SVZ cells migrate several mm to the olfactory bulb where they differentiate into neurons. These SVZ cells migrate associated with each other, forming chains of neuronal precursors (chain migration), faithfully reaching the olfactory bulb without raidal glia or axons for guidance. An extensive network for chain migration exits in the SVZ of adult mice suggesting that an unprecedented traffic of neuronal precursors exist in the adult mammalian brain. The goal is to determine the potential of the adult SVZ to generate new neurons for brain repair. To this end, the following question will be addressed. 1) Is the olfactory bulb required for the migration and proliferation of SVZ precursors? 2) Can SVZ precursors generate new neurons after transplantation into different regions of the adult brain? 3) Do chains of neuronal precursors exist in the brain of other adult vertebrates including primates and humans? 4) Which of the 5 cell types that the investigator has recently characterized in the SVZ of adult mice grow with EGF or bFGF in vitro? 5) Can SVZ precursors, either isolated directly from the brain or cultured in vitro with growth factors integrate and generate different neuronal types following transfer into the embryonic brain?