MOLECULAR DIAGNOSTIC TESTS FOR SIMIAN HERPESVIRUSES

猴子是生物医学研究的重要资源。猿猴疱疹病毒 (B病毒；BV)对工作的生物医学人员构成了非常真实的危险 和猕猴在一起。H.Papio 2(HVP2)同样会在狒狒身上引起问题 繁育的殖民地。检测受感染猴子的敏感检测方法是 需要识别对人员构成风险的动物，并识别 用于建立无病毒繁殖群体的无病毒动物。 能区分BV、HVP2和人类的敏感试验 为了准确和快速，单纯疱疹病毒(HSV)也是必要的 人类感染猴子病毒的鉴定。 将采取几种补充办法来实现这些目标。 编码BV和HVP2病毒特异性GG糖蛋白的基因将是 克隆入可诱导表达载体，建立稳定转化 细胞将被用来开发敏感的、病毒特异性的ELISA BV和HVP2抗体的检测。利用一种强大的抗体 对早期发育的病毒衣壳蛋白(P40)的反应 原发感染，BV和HSV1 p40基因将克隆到 原核表达载体用于生产重组蛋白 用于早期血清学确认的免疫分析方法的发展 人类BV感染。其他BV蛋白，它们一致地 将识别和表达猴子血清识别的基因，并表达其 对诊断测试开发的潜在用途进行了评估。单克隆 针对BV的抗体也将被生产、表征并用于 灵敏的免疫分析，以识别受感染的猴子和人类。 本项目将基础分子研究成果应用于临床。 BV和HVP2检测和诊断的重要问题。化验结果 开发将使快速、敏感和可靠的鉴别诊断成为可能 灵长类疱疹病毒感染，使灵长类群体管理者能够 有效运营设施，减少因以下原因造成的人员伤亡 人畜共患疱疹感染。

Monkeys are a vital resource for biomedical research. Herpesvirus simiae (B virus; BV) presents a very real danger for biomedical personnel who work with macaques. H. papio 2 (HVP2) similarly causes problems in baboon breeding colonies. Sensitive assays for detection of infected monkeys are needed to identify animals which pose a risk to personnel and to identify virus-free animals for establishment of virus-free breeding colonies. Sensitive assays which can differentiate between BV, HVP2 and the human herpes simplex viruses (HSVs) are also necessary for accurate and rapid identification of human infections with monkey viruses. Several complementary approaches will be pursued to achieve these goals. Genes encoding the virus-specific gG glycoproteins of BV and HVP2 will be cloned into an inducible expression vector to establish stably transformed cells which will be used to develop sensitive, virus-specific ELISAs for detection of antibody to BV and HVP2. Capitalizing on a strong antibody response to the viral capsid protein (p40) which develops early following primary infections, the BV and HSV1 p40 genes will be cloned into a procaryotic expression vector to produce recombinant proteins for development of immunoassays to provide early serological confirmation of human BV infections. Additional BV proteins which are consistently recognized by monkey sera will be identified and expressed, and their potential use for development of diagnostic tests evaluated. Monoclonal antibodies to BV will also be produced, characterized, and used for sensitive immunoassays to identify infected monkeys and humans. This project applies results of basic molecular research to the clinically important problem of BV and HVP2 detection and diagnosis. The assays developed will permit rapid, sensitive and reliable differential diagnosis of primate herpesvirus infections, enabling primate colony managers to more effectively operate facilities and reduce loss of human life due to zoonotic herpes infections.