CORE--ANTIGEN AND ANTIBODY PRODUCTION

描述：(申请人的描述)抗原的目标和 抗体生产共享资源是为客户提供及时服务 研究人员认为：1.GST-融合蛋白的生产。2.鼠标 多克隆抗血清的制备。3.小鼠单抗。4.单身 亚克隆的细胞克隆和冷冻保存。5.上述词语的使用 用于鉴定特定基因产品的试剂。 对癌症易感性、进展和预后很重要的基因有 以惊人的速度被识别。伴随这些发现的是 鉴定它们相互作用的蛋白质，这些蛋白质对 基因的功能。BRCA1和BRCA2的发现，随后， 它们与DNA修复蛋白RAD51和RAD51相互作用的证明 RAD50在乳腺癌研究中强调了这样的发现。这个 纯化融合蛋白的及时开发促进了基因研究 鉴定需要抗体的功能和产生。 特定的基因产物，免疫染色来定位蛋白质， 肿瘤标本中基因突变的筛选及其相互作用的研究 蛋白质。 这个核心将提供简化的服务，一旦一个cdna片段 获得了开放阅读框架。由于GST融合蛋白通常用作 优秀的抗原，我们将使用纯化的GST融合蛋白作为抗原 生产多克隆抗体和单抗。高质量多克隆 抗体，适用于免疫沉淀、免疫印迹和 免疫染色，将在鼠舍中产生无病原体 设施。一个完善的杂交瘤核心设施将提供服务 关于单抗产品。核心还将提供关于以下方面的建议 蛋白质产品识别和服务的方法和服务 蛋白产物的定位、免疫染色和图像分析 用特定抗体染色的细胞系或组织切片。自.以来 新发现的肿瘤抑制基因的研究或蛋白质产品， 信号分子及其相互作用的分子具有重要的 预后和诊断潜力，提供良好的特征性 及时的抗体将满足SACI的特殊需求 调查人员。

Description: (Applicant's Description) The goal of the Antigen and Antibody Production Shared Resource is to provide timely services to investigators that include: 1. GST-fusion protein production. 2. Mouse polyclonal antisera production. 3. Mouse monoclonal antibody. 4. Single cell cloning and cryopreservation of the subclone. 5. The use of above reagents in characterizing specific gene products. Genes important for cancer predisposition, progression, and prognosis are being identified at a remarkable pace. Accompanying these findings is the identification of their interacting proteins that are important for the gene function. Discoveries of BRCA1 and BRCA2, and subsequently, demonstration of their interaction with the DNA repair proteins, Rad51 and Rad50, have highlighted such discoveries in breast cancer studies. The timely development of purified fusion proteins facilitates studies of gene function and production of antibodies are required for the identification of specific gene products, immunostaining to localize the protein, screening gene mutation in cancer samples, and studies of interacting proteins. This core will provide a streamlined service once a cDNA fragment with open reading frame is obtained. Since GST-fusion proteins usually serve as excellent antigens, we will use purified GST-fusion proteins as antigens to produce polyclonal and monoclonal antibodies. High quality polyclonal antibodies, suitable for immunoprecipitation, immunoblotting and immunostaining, will be produced in mice house in the pathogen-free facility. A well-established hybridoma core facility will provide services on monoclonal antibody products. The core will also provide advice on approaches and services for protein product identification and localization of protein product, immunostaining, and image analysis of cell lines or tissue sections stained with a specific antibody. Since studies or protein products of newly identified tumor suppressor genes, signaling molecules, and their interacting molecules have important prognostic and diagnostic potential, providing well-characterized antibodies in a timely manner will meet the special needs of the SACI investigators.