ANALYSIS & PURIFICATION OF DNA IN MICROFABRICATED ARRAYS

该提案的重点是发展微加工器件， 分离和分析染色体大小的DNA分子。它将利用 在微电子工业中获得的经验， 硅基器件的微制造。 现在的设施和专业知识 存在的设计和制造，在大规模， 可用于分析、量化和 分离分子量大小范围的分子， 几千到几百万个碱基对。 对DNA电泳的理解具有重要的理论意义和 实际重要性。目前的方法局限于分离， 在合理的时间范围内，分子不大于10-7碱基 对.其原因已部分得到理解，但仍有许多问题有待解决 这是一个实践性和理论性的问题。这项建议 提出了旨在加深我们对理论理解的实验， 在制造能够分离分子的小规模装置的同时， 大小和更大的在几个小时而不是几周。因为设备是两个 三维，许多与研究聚合物相关的问题， 三个维度被规避。因为它们可以被制造出来 通过充分利用技术， 在固态工业中建立，它们是实用的， 产生大量的废化学品。

This proposal focuses on the development of microfabricated devices to separate and analyze chromosome-size DNA molecules. It will exploit the experience gained in the microelectronics industry for the microfabrication of Si-based devices. The facilities and expertise now exist for the design and manufacture, on a large scale, of microstructures that can be used for the analysis, quantification, and separation of molecules whose molecular weights range in size from a few thousand to many millions of base pairs. An understanding of DNA electrophoresis is of great theoretical and practical importance. Current methods are limited to the separation, in a reasonable time frame, of molecules no larger than 10-7 base pairs. The reasons for this are partially understood, but much remains to be done of both a practical and theoretical nature. This proposal suggests experiments designed to deepen our understanding of theory while creating small scale devices capable of separating molecules this size and larger in hours rather than weeks. Because the devices are two dimensional, many of the problems associated with studying polymers in three dimensions are circumvented. Because they can be manufactured cheaply on a large scale by taking advantage of techniques well established in the solid state industry, they are practical and do not create large quantities of spent chemicals.